dychuang@dragon.nchu.edu.tw

分子細胞生物學／生物化學

Molecular/Cellular Biology /
Biological Chemistry

1. B. S., National Chung Hsing University (1990-1994)

2. Research Student, Tohoku University (Japan) (1994-1995)

3. M. S., Tohoku University (Japan) (1995-1997)

4. Ph. D., Tohoku University (Japan) (1997-2000)

5. Postdoctoral Fellow, The University of Tokyo (Japan) (2000-2002)

6. Research Fellow, Japan Health Science Foundation (2001-2002)

7. Assistant Professor, National Chung-Hsing University (2002~2010)

8. Associate Professor, National Chung Hsing University (2010-)

細胞分化機構與訊息傳遞，基因選殖與表現，基因轉錄調控機構解析，本研究室可分成三大方向：

1. 細菌素機能與調控機構解析
細菌所生產的細菌素 (抗生物質一種 )只能殺死帶有近緣基因的細菌，而對其他親緣性較遠的物種細胞無任何的抗生效果。這種帶有特異性活性抗生物質，多數是由蛋白質所組成。近年來在針對細菌素相關研究中除了被應用在食物保存、 DNA/RNA等生物分子的修飾外、動植物病害防治外，更被發現在癌細胞的增殖或生存的抑制上有顯著的效果。本研究室主要是尋找新規的細菌素基因並加以選殖，這些新規的細菌素的殺菌活性部位、對近緣基因的細菌的辨識 (受體 )部位、細胞外進入細胞內的機構、更進一步的確認其反應機構及蛋白結構。此外，細菌素調控機構也需要進一步的研究。藉由細菌素的特性的了解，以期可以應用在醫學，工業，農業，分子生物學或各種生物防治上。

2. 細菌素胞外分泌機制解析
細菌細胞內物質分泌到細胞外需要透過分泌系統，針對分泌系統的組成不同可分別為第 I型、第 II型…等不同分泌系統，不同的分泌系統會則會分別分泌著不同的物質到細胞外，但個別分泌系統間的組成蛋白是互相獨立；我們在近年的研究中發現此一結果顯示 Erwinia屬的細菌素 Carocin S1及 Carocin S2 蛋白的胞外輸送分泌機制同時與分屬 T3aSS及 T3bSS的第三型分泌系統的部分蛋白質有間接或直接的關係。本研究目的在於探討當輸送 Carocin S1及 Carocin S2 蛋白至胞外時， T3aSS與 T3bSS分泌系統間的關係為何，其在 Erwinia屬中是否混合成一個新型態的蛋白質分泌系統以專責分泌低分子量細菌素。 Carocin S1及 Carocin S2蛋白又是如何透過此一分泌系統被輸送至胞外，這三種不同系統的蛋白彼此間的關係及其作用機制是本研究的重點。

3. 人類細胞激素多型性表現與臨床疾病關係解析
當外來病源體進入身體後體內會立即產生多種細胞激素以刺激一連串的免疫反應，藉以此抵抗病源體對身體所產生的傷害。然而需多的疾病產生有可能是與細胞激素基因表現上發生變化，導致細胞激素的活性產生變化，並造成身體的免疫障礙，進而導致疾病的產生。本研究室與基隆長庚醫院胸腔內科合作針對敗血症、肺結核。。等國人在臨床上常見的疾病進行細胞激素多型性表現分析，以從而了解國人在細胞激素多型性表現與臨床疾病關係。

(A) 期刊論文

1. Y.-C. Chan, H.–P. Wu, and D.-Y. Chuang . 2009. Extrecellular secretion of Carocin S1 in Pectobacterium carotovorum subsp. carotovorum occurs via the type III secretion system integral to the bacterial flagellum. BMC Microbiology (revised, 通訊作者)

2. H.-P. Wu, C.-K. Chen, K. Chung, J.-C. Tseng, C.-C. Hua, Y.-C. Liu, D.-Y. Chuang, C.-H. Yang. 2009. Serial Cytokine Levels in Patients with Severe Sepsis. Inflamm. Research 23:519-524 (in press, SCI= 1.602、合作論文)

3. H.-P. Wu, C.-K. Chen, K. Chung, B.-Y. Jiang, T.-J. Yu, D.-Y. Chuang. 2009. Plasma Transforming Growth Factor-β1 Level in Patients with Severe Community-acquired Pneumonia and Association with Disease Severity. J Formos Med Assoc 108:20-27 (SCI=0.5, 合作論文)

4. H.-P. Wu; C.-L. Wu; C.-K. Chen; K. Chung; J.-C. Tseng; Y.-C. Liu, and D.-Y. Chuang. 2008. The Interleukin-4 Expression in Patients with Severe Sepsis. J. Critical Care 23:519-524 (SCI=2.048, 通訊作者)

5. C-Y. Chu, C.-Y. Chen, H.-C. Yang, Y.-Y. Chiang, T.-Y. Lin, I.-P. Chiang, D.-Y. Chuang, C.-Y. Huang, C.-C. Yu, D. T. Chen, and K.-C. Chow. 2008. Expression of short form oncostatin M receptor as a decoy in lung adenocarcinomas. Journal of Pathology 215: 290-299(SCI=5.423, 合作論文)

6. D.-Y Chuang, Y.-C Chien, and H.P. Wu. 2007. Cloning and Expression of the Erwinia carotovora subsp. carotovora Gene Encoding the Low-Molecular-Weight Bacteriocin, Carocin S1, J. Bacteriol. 189: 620-629 (SCI=4.167, 第一作者、通訊作者 )

7. H.-P. Wu, C.-C. Hua, Y.-C. Liu, D.-Y. Chuang. 2007. Comparison of interferon-g response between tuberculosisand non-tubercular Pneumonia. Inflamm. Research 56: 11-16 (SCI= 1.602、合作論文 )

8. H.-P. Wu , C.-C. Hua, D.-Y. Chuang. 2007. Decreased in vitro interferon-γproduction in patients with cavitary tuberculosis on chest radiography. Respiratory Medicine 101: 48-52 (SCI=1.663、合作論文 )

9. H.-P. Wu, C.-L. Wu, C.-C. Yu, Y.-C. Liu, D.-Y. Chuang. 2007. Efficiency of interleukin-4 expression in patients with tuberculosis and nontubercular pneumonia. Human Immunology 68: 832-838(SCI=2.664, 通訊作者 )

10. Kyeremeh, A.G., Kikumoto, T., Chuang, D.Y., Gunji, Y., Takahara, Y. and Ehara, Y. 2000. Biological Control of Soft Rot of Chinese Cabbage Using Single and Mixed Treatments of Bacteriocinproducing Avirulence Mutants of Erwinia carotovora subsp. Carotovora J. Gen. Plantpathol. 66:264-270.

11. Kyeremeh, A.G., Kikumoto, T., Chuang, D.Y., Takahara, Y. and Ehara, Y. 1999. Studies on integration of bactericide-resistant biocontrol agents into conventional copper-bactericide sprays. Ann. Phytopathol. Soc. Jpn. 65: 571-575.

12. Kyeremeh, A.G., Kikumoto, T., Chuang, D.Y., Gunji, Y. and Takahara, Y. 1999. Isolation and evaluation of copper- and bactericide-resistant mutants of putative biocontrol agents of soft rot of Chinese cabbage. Ann. Phytopathol. Soc. Jpn. 65: 171-176.

13. Chuang, D.Y., Kyeremeh, A. G., Gunji, Y., Takahara, Y., Ehara, Y., and T. Kikumoto. 1999. Identification and Cloning of an Erwinia carotovora subsp. carotovora bacteriocin regulator gene by insertional mutagenesis. J. Bacteriol. 181:1953-1957.

14. Kyeremeh, A.G., Kikumoto, T., Chuang, D.Y., Gunji, Y. and Ehara, Y. 1998. Evaluation of copper- and bactericide- resistance among strains of Erwinia carotovora subsp. carotovora. Ann. Phytopathol. Soc. Jpn. 64: 198-201.

(B) 研討會論文

1. Wu, C. K., and Chuang, D. Y. 2004. Studies of Bacteriocin Genes and Structure of Erwinia carotovora subsp. Carotovora. 93 年中國化學學會年會

2. 莊敦堯, 邱富斌. 2004. Oncostatin M 在非肝細胞中誘導tyrosine amino transferase 基因中 intron A 區域的組織特異性抑制子活性. 93 年中國化學學會年會

3. Chan, Y. C.； Chuang, D. Y. 2005. Erwinia carotovora Low-Molecular-Weight Bacteriocin Protein Export Utilizes a Type Ⅲ-like Secretion System. Ann. Mol. Biol. 2005.

4. Wu, H. P., Hua, C. C., and Chuang, D. Y. 2005. Chest Radiography Patterns with IFN-Gamma and IL-4 Production in Pulmonary Tuberculosis Ann. Mol. Biol. 2005.

5. Wu, H. P., Hua, C. C., and Chuang, D. Y. 2006. Interleukin-4 Expression in Patients with Severe Sepsis Type of Presentation. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, 2006, Kyoto, Japan.

6. 詹永傑、莊敦堯. 2006. Erwinia carotovora subsp. carotovora低分子量細菌素選殖與表現 95年中國化學學會年會、台北

7. Chan, Y. C., Wu, C. L., and Chuang, D. Y. Gene Cloning and Identification of Low-Molecular-Weight Bacteriocin, carocin S2, from Erwinia carotovora subsp. carotovora. American Society for Microbiology General 107th Meeting 2007, Toronto, Canada

8. Wu, H. P., Hua, C. C., and Chuang, D. Y. 2006. Interleukin-4 Expression in Patients with Severe Sepsis Type of Presentation. American Society for Microbiology General 107th Meeting 2007, Toronto, Canada

9. Chuang, D. Y., Chan, Y. C., and Wu, C. L. Gene Cloning and Identification of Low-Molecular-Weight Bacteriocin, carocin S2, from Erwinia carotovora subsp. carotovora. 2007 Annual Meeting of the Molecular Biology Society of Japan & the Japanese Biochemical Society, Yokohama, Japan

10. Wu, H. P., Hua, C. C., Yu, C. C., Chu, C. M., Chuang, D. Y. Low Interleukin-12 Response Decrease Interferon-gamma Response in Tuberculosis Patients. 2008 Annual Meeting of the Molecular Biology Society of Japan & the Japanese Biochemical Society, Kobe, Japan

11. 黃玉珊、吳黃平、莊敦堯. 2008. The Expression of INF-γ Gene in Patients with Tuberculosis and Spesis. 97年生物醫學聯合學術年會、台北

12. 吳建利、莊敦堯. 2008. Cloning, Expression, and Purification of the CaroS2, the Immunity Protein of Carocin S2, from Erwinia carotovora. 97年生物醫學聯合學術年會、台北

13. Chan, Y-c., and Chuang, D. Y. Cloning and Expression of the Erwinia carotoovra Gene Encoding the Low-Molecular-Weight Bacteriocin Carocin S2. American Society for Microbiology General 109th Meeting 2009, Philadelphia, USA

(C) Abstracts:

1. Chuang, D. Y., Matsui, T., and Miyajima, A., 2001. Regulation of Tyrosine Amino Transferase Expression through STAT3 and C/EBPα in Fetal Liver. Workshop of Hepatocytes, 2002.

2. Chuang, D. Y., Matsui, T., Kinoshita, T., and Miyajima, A. 2001.Oncostatin M regulates expression of tyrosine amino transferase gene through STAT3 and C/EBPα. Ann. Mol. Biol. 2001.

3. Chuang, D. Y., Matsui, T., Kinoshita, T., and Miyajima, A. 2001. Regulation of Tyrosine Amino Transferase Expression by Oncostatin M in Fetal Liver. 4th IMCBNS, Taipei, Taiwan.

4. Chuang, D. Y., Matsui, T., Kinoshita, T., and Miyajima, A., 2001. Regulation of Tyrosine Amino Transferase Expression by Oncostatin M in Fetal Liver. Workshop of Hepatocytes, 2001.

5. Chuang, D.Y., Kyeremeh, A. G., and T. Kikumoto. 2000. Cloning and Expression of the Erwinia carotovora subsp. Carotovora Gene Encoding the Low-Molecular-Weight Bacteriocin, H1. Ann. Phytopathol. Soc. Jpn. 66: 138. 

6. Chuang, D.Y., Kyeremeh, A. G., and T. Kikumoto. 1998. Diverse Bacteriocins Produced by Erwinia carotovora subsp. Carotovora Ann. Phytopathol. Soc. Jpn. 64: 608-609. 

7. Chuang, D.Y., Kyeremeh, A. G., Gunji, Y., Takahara, Y., and T. Kikumoto. 1998. Cloning of Bacteriocin Regulator gene (brg) from Erwinia carotovora subsp. Carotovora. Ann. Phytopathol. Soc. Jpn. 64:389-390.

8. Kyeremeh, A.G., Chuang, D.Y., Gunji, Y., Takahara, Y., and Kikumoto, T. 1998. Isolation and Evaluation of Copper- and Bacteriocide- resistant Mutants of Putative Biocontrol Agents of the Soft-rot Disease of Chinese Cabbage. Ann. Phytopathol. Soc. Jpn. 64: 385. 

9. Kikumoto, T., Kyeremeh, A. G., Chuang, D. Y., and Gunji, Y. 1997. Biological control of the soft rot disease of Chinese cabbage with avirulent mutant strains of Erwinia carotovora subsp. Carotovora. Proc. 4th Intl. Workshop on PGPR, Japan-OECD Joint Workshop, Sapporo, pp. 118-119.

10. Kyeremeh, A.G., Chuang, D.Y., Gunji, Y., and Kikumoto, T. 1996. Copper-resistance in Erwinia carotovora subsp. Carotovora. Ann. Phytopathol. Soc. Jpn. 62: 605.

(D) Preparation Articles:

1. Chuang, D. Y., Kyeremeh, A. G., and Kikumoto, T. Induction of Bacteriocin Synthesis in Erwinia carotovora subsp. Carotovora is controlled by the expression of flhDC. (Preparation)

2. Chuang, D. Y., Kyeremeh, A. G., and Kikumoto, T. Cloning and Expression of Erwinia carotovora subsp. carotovora Gene Encoding the Low-Molecular-Weight Bacteriocin, Carocin H1. (Preparation)

3. Chuang, D. Y., and Kikumoto, T. New Induction and Regulatory Mechanism of the Low-Molecular-Weight Bacteriocin Expression in Erwinia carotovora subsp. carotovora. (Preparation)

4. Chuang, D. Y., Matsui, T., and Miyajima, A. Cooperation of STAT3 and C/EBPα for expression of the tyrosine amino transferase gene in mouse fetal hepatocytes: identification of an Oncostatin M responsive element. (Preparation)

國立中興大學化學系National Chung Hsing University Department of Chemistry

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