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hhhsiao@dragon.nchu.edu.tw

生物化學

Biological Chemistry

 

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1.          B. S., Department of Chemistry, National Chung Hsing University (1995-1999)

2.          M. S., Department of Chemistry, National Chung Hsing University (1999-2001)

3.          Ph. D., Biology Faculty, Georg-August-Universität Göttingen, Germay (2007-2010)

4.          Research Assistant, Institute of Biological Chemistry, Academia Sinica, Taiwan (2001-2007)

5.          Postdoctoral , Max Planck Institute for Biophysical Chemistry, Germany (2010-2012)

6.          Assistant Professor, National Chung Hsing Normal University (2012-present)

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蛋白質體學、生物質譜分析

主要研究重心為利用層析分離方法與生物質譜分析技術,並結合蛋白質資料庫搜尋,來進行大規模的蛋白質身份鑑定及轉譯後修飾之偵測,例如:蛋白質磷酸化、醣基化、泛素化等。此外可藉由紫外光照射核糖核酸與蛋白質分子,來形成穩定交叉結合錯合物,進而探討生物分子之間的交互作用。並可經由穩定同位素標定來進行蛋白質之定量分析,結合米粒子科技純化蛋白質及肽,研究抗癌藥物與蛋白質之間的交互作用,尋找新的疾病指標,作為早期疾病診斷依據。

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Proteomics and Bioanalytical Mass Spectrometry

The primary focus of the research is the large scale study of protein identification and protein post-translational modifications (PTMs), such as protein phosphorylation, glycosylation and ubiquitination and so on, by the tools of electrophoresis, chromatography and bioanalytical mass spectrometry in combination with protein database searching. We are also engaged in the study of protein-RNA interactions by utilizing UV-induced crosslinking of protein-RNA complexes. Furthermore, protein quantifications, protein-protein interactions, protein-drug interactions, biomarker discovery and disease diagnosis are investigated by means of stable isotope labeling technology and affinity purification of nanoparticles.

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1.      Borsarelli C. D.*, Falomir-Lockhart L. J., Ostatna´ V., Fauerbach J. A., Hsiao H. H., Urlaub H., Palecˇek E., Jares-Erijman E. A., Jovin T. M. (2012) “Biophysical properties and cellular toxicity of covalent crosslinked oligomers of a-synuclein formed by photoinduced side-chain tyrosyl radicals” Free Radical Biology and Medicine, In Press. (Impact Factor: 5.423)

2.      Thorn, A., Steinfeld, R., Ziegenbein, M., Grapp, M., Hsiao, H. H., Urlaub, H., Sheldrick, G. M., Gartner, J.*, Kratzner, R.* (2012). "Structure and activity of the only human RNase T2." Nucleic acids research, In Press. (Impact Factor: 8.026)

3.      Himmel, S., Zschiedrich, C. P., Becker, S., Hsiao, H. H., Wolff, S., Diethmaier, C., Urlaub, H., Lee, D., Griesinger, C.*, Stulke, J.* (2012). "Determinants of interaction specificity of the Bacillus subtilis GlcT antitermination protein: Functionality and phosphorylation specificity depend on the arrangement of the regulatory domains." JOURNAL OF BIOLOGICAL CHEMISTRY, In Press. (Impact Factor: 4.773)

4.      Hsiao, H. H., Meulmeester, E., Urlaub, H.* (2012). "Identification of Endogenous SUMO1 Accepter Sites by Mass Spectrometry." Methods in molecular biology 893, 431-441. (Book Chapter)

5.      Kuo, C. W., Wu, I. L., Hsiao, H. H., Khoo, K. H.* (2012). "Rapid glycopeptide enrichment and N-glycosylation site mapping strategies based on amine-functionalized magnetic nanoparticles." Analytical and bioanalytical chemistry 402(9), 2765-2776. (Impact Factor: 3.778)

6.      Oellerich T., Bremes V., Neumann K., Bohnenberger H., Dittmann K., Hsiao H. H., Engelke M., Schnyder T., Batista F., Urlaub H.*, Wienands J.* (2011)The B-cell antigen receptor signals through a preformed transducer module of SLP65 and CIN85” EMBO Journal 30 (17), 3620-3634. (Impact Factor: 9.205)

7.      Krumova P., Meulmeester E., Garrido M., Tirard M., Hsiao H. H., Bossis G., Urlaub H., Zweckstetter M., Kügler S., Melchior F.*, Bähr M., Weishaupt J. H.* (2011) “Sumoylation regulates α-synuclein aggregation and toxicity.” Journal of Cell Biology 194 (1), 49-60. (Impact Factor: 10.264)

8.      Kramer K., Hummel P., Hsiao H. H., Luo X., Wahl M., Urlaub H.* (2011) “Mass-spectrometric analysis of proteins cross-linked to 4-thio-uracil- and 5-bromo-uracil-substituted RNA.” International Journal of Mass Spectrometry 304 (2-3), 184-194. (Impact Factor: 2.549)

9.      Yeh Y. Y., Shah K. H., Chou C. C., Hsiao H. H., Wrasman K. M., Stephan J. S., Stamatakos D., Khoo K. H., Herman P. K.* (2011) “The identification and analysis of phosphorylation sites on the Atg1 protein kinase.” Autophagy 7 (7), 716-726. (Impact Factor: 7.453)

10.  Rüdel S., Wang Y., Lenobel R., Körner R., Hsiao H. H., Urlaub H., Patel D., Meister G.* (2011) “Phosphorylation of human Argonaute proteins affects small RNA binding.” NUCLEIC ACIDS RESEARCH 39 (6), 2330-2043. (Impact Factor: 8.026)

11.  Bohnenberger, H., Oellerich, T., Engelke, M., Hsiao, H. H., Urlaub, H., Wienands, J.* (2011) “Complex phosphorylation dynamics control the composition of the Syk interactome in B cells.” European Journal of Immunology 41 (6), 1550-1562. (Impact Factor: 5.103)

12.  Ghalei, H., Hsiao, H.H., Urlaub, H., Wahl, M.C., Watkins NJ.* (2010) “A novel Nop5-sRNA interaction that is required for efficient archaeal box C/D sRNP formation.” RNA 16 (12), 2341-2348. (Impact Factor: 5.095)

13.  Beller M., Bulankina A. V., Hsiao H. H., Urlaub H., Jäckle H., Kühnlein R. P.* (2010) “PERILIPIN-dependent control of lipid droplet structure and fat storage in Drosophila.” Cell Metabolism 12 (5), 521-532. (Impact Factor: 13.668)

14.  Hsiao, H. H., Urlaub, H.* (2010) “Pseudo-Neutral-Loss Scan for Selective Detection of Phosphopeptides and N-Glycopeptides using Liquid Chromatography Coupled with a Hybrid Linear Ion-Trap / Orbitrap Mass Spectrometer.” PROTEOMICS 10 (21), 3916-3921. (Impact Factor: 4.505)

15.  Kuhn-Holsken, E., Lenz, C., Dickmanns, A., Hsiao, H. H., Richter, F. M., Kastner, B., Ficner, R., and Urlaub, H.* (2010) “Mapping the binding site of snurportin 1 on native U1 snRNP by cross-linking and mass spectrometry.” NUCLEIC ACIDS RESEARCH 38 (16), 5581-5593. (Impact Factor: 8.026)

16.  Wu, H. J., Seib, K. L., Srikhanta, Y. N., Edwards, J., Kidd, S. P., Maguire, T. L., Hamilton, A., Pan, K. T., Hsiao, H. H., Yao, C. W., Grimmond, S. M., Apicella, M. A., McEwan, A. G., Wang, A. H., and Jennings, M. P.* (2010) “Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae.” JOURNAL OF PROTEOMICS 73 (5), 899-916. (Impact Factor: 4.878)

17.  Schneider, M., Hsiao, H. H., Will, C. L., Giet, R., Urlaub, H., and Luhrmann, R.* (2010) “Human PRP4 kinase is required for stable tri-snRNP association during spliceosomal B complex formation.” NATURE STRUCTURAL & MOLECULAR BIOLOGY 17 (2), 216-221. (Impact Factor: 12.712)

18.  Hsiao, H. H., Meulmeester, E.*, Frank, B. T., Melchior, F., and Urlaub, H.* (2009) “"ChopNSpice," a mass spectrometric approach that allows identification of endogenous small ubiquitin-like modifier-conjugated peptides.” MOLECULAR & CELLULAR PROTEOMICS 8 (12), 2664-2675. (Impact Factor: 7.398)

19.  Yu, S. Y., Wu, S. W., Hsiao, H. H., and Khoo, K. H.* (2009) “Enabling techniques and strategic workflow for sulfoglycomics based on mass spectrometry mapping and sequencing of permethylated sulfated glycans.” GLYCOBIOLOGY 19 (10), 1136-1149. (Impact Factor: 3.580)

20.  Oellerich, T., Gronborg, M., Neumann, K., Hsiao, H. H., Urlaub, H.*, and Wienands, J.* (2009) “SLP-65 phosphorylation dynamics reveals a functional basis for signal integration by receptor-proximal adaptor proteins.” MOLECULAR & CELLULAR PROTEOMICS 8 (7), 1738-1750. (Impact Factor: 7.398)

21.  Martin Richter, F., Hsiao, H. H., Plessmann, U., and Urlaub, H.* (2009) “Enrichment of protein-RNA crosslinks from crude UV-irradiated mixtures for MS analysis by on-line chromatography using titanium dioxide columns.” BIOPOLYMERS 91 (4), 297-309. (Impact Factor: 2.870)

22.  Luo, X., Hsiao, H. H., Bubunenko, M., Weber, G., Court, D. L., Gottesman, M. E.*, Urlaub, H., and Wahl, M. C.* (2008) “Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.” MOLECULAR CELL 32 (6), 791-802. (Impact Factor: 14.178)

23.  Meulmeester, E., Kunze, M., Hsiao, H. H., Urlaub, H., and Melchior, F.* (2008) “Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25.” MOLECULAR CELL 30 (5), 610-619. (Impact Factor: 14.178)

24.  Hsiao, H. H., Hsieh, H. Y., Chou, C. C., Lin, S. Y., Wang, A. H., and Khoo, K. H.* (2007) “Concerted experimental approach for sequential mapping of peptides and phosphopeptides using C18-functionalized magnetic nanoparticles.” JOURNAL OF PROTEOME RESEARCH 6 (4), 1313-1324. (Impact Factor: 5.113)

25.  Chen, Y. Y., Lin, S. Y., Yeh, Y. Y., Hsiao, H. H., Wu, C. Y., Chen, S. T., and Wang, A. H.* (2005) “A modified protein precipitation procedure for efficient removal of albumin from serum.” ELECTROPHORESIS 26 (11), 2117-2127. (Impact Factor: 3.303)

26.  Tsai, J. M., Wang, H. C., Leu, J. H., Hsiao, H. H., Wang, A. H., Kou, G. H., and Lo, C. F.* (2004) “Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus.” JOURNAL OF VIROLOGY 78 (20), 11360-11370. (Impact Factor: 5.402)

27.  Chung, T. L., Hsiao, H. H., Yeh, Y. Y., Shia, H. L., Chen, Y. L., Liang, P. H., Wang, A. H., Khoo, K. H.*, and Shoei-Lung Li, S.* (2004) “In vitro modification of human centromere protein CENP-C fragments by small ubiquitin-like modifier (SUMO) protein: definitive identification of the modification sites by tandem mass spectrometry analysis of the isopeptides.” JOURNAL OF BIOLOGICAL CHEMISTRY 279 (38), 39653-39662., Equal contribution as the first author. (Impact Factor: 4.773)

28.  Lee, C. L., Hsiao, H. H., Lin, C. W., Wu, S. P., Huang, S. Y., Wu, C. Y., Wang, A. H.*, and Khoo, K. H. (2003) “Strategic shotgun proteomics approach for efficient construction of an expression map of targeted protein families in hepatoma cell lines.” PROTEOMICS 3 (12), 2472-2486. (Impact Factor: 4.505)

29.  蕭鶴軒, 李茂榮. (2000) “吹氣捕捉萃取技術.” 科儀新知, 119, 22, 3, 83-91

 

國立中興大學化學系National Chung Hsing University Department of Chemistry

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