楊文明 教授 Wen-Ming Yang,PhD                   BACK




美國德州大學博士
個人網頁
http://web.nchu.edu.tw/~yangwm/
http://web.nchu.edu.tw/~yangwm/papers.htm
yangwm@mail.nchu.edu.tw

yangwm@email.nchu.edu.tw

辦公室電話: 04-22840485#246
實驗室電話: 04-22840485#247

 
簡要經歷
 
1. 博士(美國德州大學,健康科學中心,聖安東尼分校,分子醫學研究所)
2. 學士 (國立中興大學)

經歷
1. 副教授 (分子生物學研究所,國立中興大學)
2. 助理教授 (分子生物學研究所,國立中興大學)
3. 博士後研究員(瑪菲特癌症腫瘤研究中心,美國南佛羅里達州大學

研究方向
 


從基因與蛋白分子的角度來探求人類重大疾病以及癌症的形成原因,並研究發展相關蛋白與可能藥物的關係,以期建立癌症與人類重大疾病預防與治療之可行性。

近期參與之研究計劃:

  1. 許多人類的重大疾病往往是由於細胞本身受到不正常的控制所成。其中控制細胞正常運作的是由一些調控蛋白所主導。主導的過程又以基因轉錄 (Transcription) 最為重要。基因轉錄的程度是受到轉錄調控蛋白 (Transcription factors) 所控制。而在人類細胞中,許多的轉錄調控蛋白會和其它重要蛋白分子形成蛋白複合體 (Protein complexes)。這些蛋白複合體的功能和分子機制都一無所知。 目前,我們從人類癌細胞中分離純化出一些和癌症相關的蛋白複合體, 期待能從這些癌細胞的成員中了解癌症重大疾病形成的分子機制,並發展出可能的預防與治療藥物。
  2.  

  3. 耳聾是一種相當常見的疾病。據統計,每八百位在新生兒中就有一位患有先天全聾或是嚴重的聽力障礙。也有為數不少是在成長中漸進發病的。造成耳聾的原因不外是環境因素和基因缺陷。然而,尋找與耳聾相關的基因是相當困難的,特別是無相關症候群型的耳聾 (non-syndromic deafness)。過去十年中,利用分子遺傳學的技術,已經發現70個染色體的位置和人類耳聾有關。這樣的發現也同時說明了耳聾是一種非常複雜的疾病,而有更多未知的相關基因等待去發現。去年,由於人類的基因完全解碼,加上小鼠為實驗的成功動物模式,許多和耳聾相關的重要基因都被發現。但是,我們都了解要從發現一個基因到了解一個基因的功能,永遠是最重要,也是一定要做要走的一步。因此,我們在想,在這些和耳聾有關的基因中,哪些可以幫助我們了解聽力好壞的分子機制﹖從蛋白質體學的方法及原理來研分子間的交互作用是可以解決回答這些問題的。

 

實驗室概況:

  1. 研究生之一從事人類疾病 Waardenburg Syndrome 的研究。

  2. 研究生之二從事癌細胞轉移 (Metastasis) 的分子機制研究。

  3. 研究生之三從事細胞週期 (The Cell Cycle) 相關蛋白之分子機制研究。

  4. 研究生之四從事 Heterochromatin and HDACs的研究。

  5. 研究生之五從事 Zinc Finger Repressors 的研究。

  6. 研究助理從事 HDACs 相關蛋白之分子機制研究。

  7. 博士後研究員從事癌細胞蛋白複合體與抗癌藥物的分子機制研究。

  8. 大學部研究生從事細胞核與基因表現的研究。

  9. 期待對基礎研究有熱情,不畏艱難,穩紮穩打的年輕新生命,加入我們尖端的研究。



近期著作
 


  1. Loading of PAX3 to Mitotic Chromosomes Is Mediated by Arginine Methylation and Associated with Waardenburg Syndrome.
    Wu TF, Yao YL, Lai IL, Lai CC, Lin PL, Yang WM*.
    J Biol Chem. 2015 Aug 14;290(33):20556-64.
  2. PAX3 Loads onto Pericentromeric Heterochromatin During S Phase Through PARP1.
    Wu TF, Yao YL, Lai IL, Lee TH, Underhill DA, Yang WM.
    Anticancer Res. 2014 Sep;34(9):4717-22.
  3. The transcriptional repression activity of STAF65γ is facilitated by promoter tethering and nuclear import of class IIa histone deacetylases.
    Hsieh FS, Chen NT, Yao YL, Wang SY, Chen JJ, Lai CC, Yang WM*.
    Biochim Biophys Acta. 2014 Jul;1839(7):579-91. doi: 10.1016/j.bbagrm.2014.05.007. Epub 2014 May 19.
  4. Loss of ZBRK1 Contributes to the Increase of KAP1 and Promotes KAP1-Mediated Metastasis and Invasion in Cervical Cancer.
    Lin LF, Li CF, Wang WJ, Yang WM, Wang DD, Chang WC, Lee WH, Wang JM.
    PLoS One. 2013 Aug 22;8(8):e73033. doi: 10.1371/journal.pone.0073033.
  5. PARP-2 regulates cell cycle-related genes through histone deacetylation and methylation independently of poly(ADP-ribosyl)ation.
    Liang YC, Hsu CY, Yao YL, Yang WM.
    Biochem Biophys Res Commun. 2013 Feb 1;431(1):58-64. doi: 10.1016/j.bbrc.2012.12.092. Epub 2013 Jan 3.
  6. FKBPs in chromatin modification and cancer.
    Yao YL, Liang YC, Huang HH, and Yang, W. M.*
    Curr Opin Pharmacol. 2011 Aug;11(4):301-7. Epub 2011 Apr 12
  7. Beyond histone and deacetylase: an overview of cytoplasmic histone deacetylases and their nonhistone substrates.
    Yao YL, Yang WM.
    J Biomed Biotechnol. 2011;2011:146493. Epub 2010 Dec 22.
  8. HDAC10 relieves repression on the melanogenic program by maintaining the deacetylation status of repressors. Lai IL, Lin TP, Yao YL, Lin CY, Hsieh MJ, and Yang, W. M.*
    J Biol Chem. 2010 Mar 5;285(10):7187-96. Epub 2009 Dec 22.
  9. Sumoylation of LAP1 is involved in the HDAC4-mediated repression of COX-2 transcription.
    Wang WL, Lee YC, Yang, W. M., Chang WC, Wang JM.
    Nucleic Acids Res. 2008, 36(19): 6066-6079.
  10. HDAC1/HDAC3 modulates PPARG2 transcription through the sumoylated CEBPD in hepatic lipogenesis.
    Lai PH, Wang WL, Ko CY, Lee YC, Yang, W. M., Shen TW, Chang WC, Wang JM.
    Biochim Biophys Acta. 2008, 1783(10): 1803-1814.
  11. Transcriptional and subcellular regulation of the TRIP-Br family.Lai, I. J., Wang, S. Y., Yao, Y. L. and Yang, W. M.*Gene. 2007, 388 (1-2): 102-109.
  12. Transcriptional repression activity of PAX3 is modulated by competition between corepressor KAP1 and heterochromatin protein 1. Hsieh, M. J., Yao, Y. L., Lai, I. L., and Yang, W. M. *
    Biochem Biophys Res Commun. 2006, 349(2): 573-581.
  13. Nuclear proteins: promising targets for cancer drugs.
    Yao Y. L. and Yang W. M.
    Curr Cancer Drug Targets. 2005, 5(8): 595-610. (Impact factor: 5.67 for year 2007)
  14. The transcriptional factor YY1 upregulates the novel invasion suppressor HLJ1 expression and inhibits cancer cell invasion.Wang CC, Tsai MF, Hong TM, Chang GC, Chen CY, Yang WM, Chen JJ, Yang PC.
    Oncogene 2005, 24(25): 4081-4093.
  15. Histone deacetylases: purification of the enzymes, substrates, and assayconditions.Rezai-Zadeh N, Tsai SC, Wen YD, Yao YL, Yang WM, Seto EMethods Enzymol 2004, 377:167-79
  16. The metastasis-associated proteins 1 and 2 form distinct protein complexes with histone deacetylase activity.Yao YL, Yang WMJ Biol Chem 2003 Oct 24, 278:42560-8. (Selected as Breaking News pubished in Leaddiscovery)
  17. Functional domains of histone deacetylase-3.Yang WM, Tsai SC, Wen YD, Fejer G, Seto EJ Biol Chem 2002, 277 :9447-9454.
  18. NAPP2, a Peroxisomal Membrane Protein, Is Also a Transcriptional Corepressor.Gavva NR, Wen SC, Daftari P, Moniwa M, Yang WM , Yang-Feng LP, Seto E, Davie JR, Shen CKGenomics 2002, 79:423-31
  19. Yin-Yang 1 activates interleukin-4 gene expression in T cells.Guo J, Casolaro V, Seto E, Yang WM, Chang C, Seminario MC, Keen J, Georas SNJ Biol Chem 2001, 276:48871-8
  20. Histone deacetylase activity represses gamma interferon-inducible HLA-DR gene expression following the establishment of a DNase I-hypersensitive chromatin conformation.Osborne A, Zhang H, Yang WM, Seto E, Blanck GMol Cell Biol 2001, 21:6495-506
  21. The FK506-binding protein 25 functionally associates with histone deacetylases and with transcription factor YY1.Yang WM, Yao YL, Seto EEMBO J 2001, 20:4814-25
  22. Regulation of transcription factor YY1 by acetylation and deacetylation.Yao YL, Yang WM, Seto E
    Mol Cell Biol 2001, 21:5979-91
  23. The growth suppressor PML represses transcription by functionally and physically interacting with histone deacetylases.Wu WS, Vallian S, Seto E, Yang WM, Edmondson D, Roth S, Chang KSMol Cell Biol 2001, 21:2259-68
  24. Histone deacetylase interacts directly with DNA topoisomerase II.Tsai SC, Valkov N, Yang WM, Gump J, Sullivan D, Seto ENat Genet 2000, 26:349-53
  25. The histone deacetylase-3 complex contains nuclear receptor corepressors.Wen YD, Perissi V, Staszewski LM, Yang WM, Krones A, Glass CK, Rosenfeld MG, Seto EProc Natl Acad Sci U S A 2000, 97:7202-7
  26. Histone deacetylases specifically down-regulate p53-dependent gene activation.Juan LJ, Shia WJ, Chen MH, Yang WM, Seto E, Lin YS, Wu CWJ Biol Chem 2000, 275:20436-43
  27. Ligand-induced recruitment of a histone deacetylase in the negative-feedback regulation of the thyrotropin beta gene.Sasaki S, Lesoon-Wood LA, Dey A, Kuwata T, Weintraub BD, Humphrey G, Yang WM, Seto E, Yen PM, Howard BH, Ozato KEMBO J 1999, 18:5389-98
  28. RBP1 recruits both histone deacetylase-dependent and -independent repression activities to retinoblastoma family proteins.Lai A, Lee JM, Yang WM, DeCaprio JA, Kaelin WG, Seto E, Branton PE
    Mol Cell Biol 1999, 19:6632-41
  29. Cloning and characterization of the mouse histone deacetylase-2 gene.Zeng Y, Tang CM, Yao YL, Yang WM, Seto EJ Biol Chem 1998, 273:28921-30
  30. Isolation and characterization of cDNAs corresponding to an additional member of the human histone deacetylase gene family.Yang WM, Yao YL, Sun JM, Davie JR, Seto EJ Biol Chem 1997, 272:28001-7
  31. The hepatitis B virus X-associated protein, XAP3, is a protein kinase C-binding protein.Cong YS, Yao YL, Yang WM, Kuzhandaivelu N, Seto EJ Biol Chem 1997, 272:16482-9
  32. Differential effects of nuclear receptor corepressor (N-CoR) expression levels on retinoic acid receptor-mediated repression support the existence of dynamically regulated corepressor complexes.Soderstrom M, Vo A, Heinzel T, Lavinsky RM, Yang WM, Seto E, Peterson DA, Rosenfeld MG, Glass CK Mol Endocrinol 1997, 11:682-92
  33. Histone deacetylases associated with the mSin3 corepressor mediate mad transcriptional repression.
    Laherty CD, Yang WM, Sun JM, Davie JR, Seto E, Eisenman RN Cell 1997, 89:349-56
  34. A complex containing N-CoR, mSin3 and histone deacetylase mediates transcriptional repression.
    Heinzel T, Lavinsky RM, Mullen TM, Soderstrom M, Laherty CD, Torchia J, Yang WM, Brard G, Ngo SD, Davie JR, Seto E, Eisenman RN, Rose DW, Glass CK, Rosenfeld MGNature 1997, 387:43-8
  35. XAP2, a novel hepatitis B virus X-associated protein that inhibits X transactivation.Kuzhandaivelu N, Cong YS, Inouye C, Yang WM, Seto ENucleic Acids Res 1996, 24:4741-50
  36. Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3.Yang WM, Inouye C, Zeng Y, Bearss D, Seto EProc Natl Acad Sci U S A 1996, 93:12845-50
  37. Cyclophilin A and FKBP12 interact with YY1 and alter its transcriptional activity.Yang WM, Inouye CJ, Seto EJ Biol Chem 1995, 270:15187-93

可由 http://web.nchu.edu.tw/~yangwm/papers.htm查詢



專  利
 

榮  譽
   
授課課程
 
1. Eukaryotic Gene Transcription (in English)
2. Epigenetics (in English)
3. Cell Proliferation & Apoptosis (Cell Fates) (in English)
4. The Nucleus (in English)
5. (Advanced) Molecular Cell Biology
6. 分子生物學技術(一)、(二)
7. 文獻研討 (in English)
8. 專題討論 (in English)
9. 專題研究 (in English