楊秋英 教授Chiou-Ying Yang                  BACK




美國波士頓大學博士
cyyang@dragon.nchu.edu.tw
辦公室電話: 04-22840485#241
實驗室電話: 04-22840485#242



簡要經歷
 
1. 中興大學助教
2. 美國哈佛大學醫學院Research Associate
3. 美國波士頓大學博士
4. 中興大學副教授
5. 國家衛生研究院兼任副研究員(95年6月~~103年12月)
6. 中興大學教授
7. 中興大學分子生物學研究所所長(105年8月~迄今)
研究方向與計畫
 
一、

細菌疫苗的研發:

 
  1. 疫苗抗原的鑑定與功能探討


  2. 檢驗與治療用抗體的開發


二、 近五年來主持之研究計畫:
 
  1. 鮑氏不動桿菌上具有保護力之B細胞抗原的鑑定(國科會2012-2016)


  2. 建構偵測污染食品微生物的微型化全分析快速篩檢平台—以沙門氏菌檢測為例--子計畫二:製備食物中毒病原菌抗體與適當之分子探針(國科會2012-2015)


  3. 沙門氏桿菌之重組疫苗研發(農委會-家衛所 2010-2013)


  4. 以免疫蛋白質體法分析食物毒害細菌中之金黃葡萄球菌(國科會2009-2012)


近期著作
 
A、

期刊論文:(*corresponding author)

 
  1. Lin HY, Huang CH, Hsieh WH, Liu LH, Lin YC, Chu CC, Wang ST, Kuo IT, Chau LK*, Yang CY*. On-line SERS detection of single bacterium using novel SERS nanoprobes and a microfluidic dielectrophoresis device. Small. 2014; 10:4700-10.


  2. Sharon J, Rynkiewicz MJ, Lu Z, Yang CY. Discovery of protective B-cell epitopes for development of antimicrobial vaccines and antibody therapeutics. Immunology. 2014; 142:1-23. Review.

  3. Lu Z, Rynkiewicz MJ, Madico G, Li S, Yang CY, Perkins HM, Sompuram SR, Kodela V, Liu T, Morris T, Wang D, Roche MI, Seaton BA, Sharon J. B-cell epitopes in GroEL of Francisella tularensis. PLoS One. 2014; 9:e99847.

  4. Lu Z, Rynkiewicz MJ, Yang CY, Madico G, Perkins HM, Roche MI, Seaton BA, Sharon J. Functional and structural characterization of Francisella tularensis O-antigen antibodies at the low end of antigen reactivity. Monoclon Antib Immunodiagn Immunother. 2014; 33:235-45.

  5. Lu Z, Rynkiewicz MJ, Yang CY, Madico G, Perkins HM, Wang Q, Costello CE, Zaia J, Seaton BA, Sharon J. The binding sites of monoclonal antibodies to the non-reducing end of Francisella tularensis O-antigen accommodate mainly the terminal saccharide. Immunology. 2013; 140:374-89.

  6. Ko CF, Lin NT, Chiou CS, Wang LY, Liu MC, Yang CY, Lee YS. Infrequent cross-transmission of Shigella flexneri 2a strains among villages of a mountainous township in Taiwan with endemic shigellosis. BMC Infect Dis. 2013; 13:354.

  7. Chu CL, Yu YL, Kung YC, Liao PY, Liu KJ, Tseng YT, Lin YC, Hsieh SS, Chong PC, Yang CY*. The immunomodulatory activity of meningococcal lipoprotein Ag473 depends on the conformation made up of the lipid and protein moieties. PLoS One. 2012; 7:e40873.

  8. Luo CH, Chiou PY, Yang CY, Lin NT. Genome, integration, and transduction of a novel temperate phage of Helicobacter pylori. J Virol. 2012;86:8781-92.

  9. Chen CH, Lin NT, Hsiao YM, Yang CY*, Tseng YH*. Two non-consensus Clp binding sites are involved in upregulation of the gum operon involved in xanthan polysaccharide synthesis in Xanthomonas campestris pv. campestris. Res Microbiol. 2010; 161:583-9.

  10. Sung JW, Hsieh SY, Lin CL, Leng CH, Liu SJ, Chou AH, Lai LW, Lin LH, Kwok Y, Yang CY, Chong P. Biochemical characterizations of Escherichia coli-expressed protective antigen Ag473 of Neisseria meningitides group B. Vaccine. 2010; 28:8175-82.

  11. Chen HW, Liu SJ, Liu HH, Kwok Y, Lin CL, Lin LH, Chen MY, Tsai JP, Chang LS, Chiu FF, Lai LW, Lian WC, Yang CY, Hsieh SY, Chong P, Leng CH. A novel technology for the production of a heterologous lipoprotein immunogen in high yield has implications for the field of vaccine design. Vaccine. 2009; 27:1400-9.

  12. Hsu CA, Lin WR, Li JC, Liu YL, Tseng YT, Chang CM, Lee YS, Yang CY*. Immunoproteomic identification of the hypothetical protein NMB1468 as a novel lipoprotein ubiquitous in Neisseria meningitidis with vaccine potential. Proteomics. 2008; 8:2115-25. doi: 10.1002/pmic.200700574.

  13. Yang CY*, Lee YS, Huang LS, Kuo YL, Liu YL, Lu CH. Antigenic diversity of Neisseria meningitidis isolated in Taiwan between 1995 and 2002. Scand J Infect Dis. 2006; 38:273-80.

  14. Wiberg FC, Rasmussen SK, Frandsen TP, Rasmussen LK, Tengbjerg K, Coljee VW, Sharon J, Yang CY, Bregenholt S, Nielsen LS, Haurum JS, Tolstrup AB. Production of target-specific recombinant human polyclonal antibodies in mammalian cells. Biotechnol Bioeng. 2006; 94:396-405.

  15. Sharon J, Sompuram SR, Yang CY, Williams BR, Sarantopoulos S. Construction of polyclonal antibody libraries using phage display. Methods Mol Biol. 2002;178:101-12. Review.

  16. Chen L, Williams BR, Yang CY, Cevallos AM, Bhat N, Ward H, Sharon J. Polyclonal Fab phage display libraries with a high percentage of diverse clones to Cryptosporidium parvum glycoproteins. Int J Parasitol. 2003; 33:281-91.

  17. Lee YS, Yang CY, Lu CH, Tseng YH. Molecular epidemiology of Bordetella pertussis isolated in Taiwan, 1992-1997. Microbiol Immunol. 2003; 47:903-9.

  18. Hung CH, Yang CF, Yang CY, Tseng YH. Involvement of tonB-exbBD1D2 operon in infection of Xanthomonas campestris phage phi L7. Biochem Biophys Res Commun. 2003; 302:878-84.

  19. Sharon J, Sarantopoulos S, Den W, Kao CYY, Baecher-Allan CM, Santora KE, Sompuram SR, Petersen-Mahrt S, Williams BR. Recombinant polyclonal antibody libraries. Comb Chem High Throughput Screen. 2000;3:185-96. Review.

  20. Sarantopoulos S, Kao CYY, Den W, Sharon J. A method for linking VL and VH region genes that allows bulk transfer between vectors for use in generating polyclonal IgG libraries. J Immunol. 1994;152:5344-51.

  21. Kao CYY, Sharon J. Chimeric antibodies with anti-dextran-derived complementarity-determining regions and anti-p-azophenylarsonate-derived framework regions. J Immunol. 1993 ;151:1968-78.

  22. Sharon J, Kao CYY, Sompuram SR. Oligonucleotide-directed mutagenesis of antibody combining sites. Int Rev Immunol. 1993;10:113-27. Review.

B. 研討會論文(2011-2015):
 
  1. Hung CC, Lin Y, Y CY. *. 2011. Spa protein is found to be the most abundant extracellular protein in a food-borne Staphylococcus aureus. The 111th General Meeting of American Society for Microbiology.


  2. Sharon J, Lu Z, Rynkiewicz MJ, Madico G, Wang Q, Sompuram SR, Liu T, Yang CY, Roche MI, Perkins HM, Hui JH,. Kodela V, Morris T, Li S, Wang D, Seaton BA, Zaia J, Costello CE, Woods VL Jr. 2012. Protective B cell epitopes in tularemia. The 7th International Conference on Tularemia, Breckenridge, CO, Sept 17-20, 2012.


  3. Yang ZH, Kung YC, Yang CY *. 2015. Characterization of the Acinetobacter baumannii glycoprotein A1S_3744. 2015 International Congress on Natural Sciences and Engineering, May 07-09, Kyoto, Japan.


C. 專書或書中章節:
 
  1. Sharon, J., S. R. Sompuram, C.-Y. Yang, B. R. Williams, and S. Sarantopoulos. 2001. Construction of Polyclonal Antibody Libraries Using Phage Display. Methods in Molecular Biology vol 178: Antibody Phage Display: Methods and Protocols Edited by: P.M. O'Brien and R. Aitken, Humana Press Inc., Totowa, NJ pp 101-112 (Chapter 6).


  2. 楊秋英、林台珮、曾彥慈、陳良築。 建立水稻為腦膜炎雙球菌抗體之生產平台。 「作物基因轉移技術之開發應用」計畫成果研討會專刊139-152頁;曾夢蛟 黃鵬林 主編 (2005)。

  3. 楊秋英。由學術通往產業契機的故事:腦膜炎雙球菌次單元疫苗。『以啟山林:國衛院疫苗研發中心發展旅程』蘇益仁等合著(2013)。


專  利
 


專利:

  1. Polyclonal Antibody Libraries- Patent No. 5,789208 (scientific collaborator)


  2. Method for manufacturing recombinant polyclonal proteins / 15830830 / European


  3. Surface protein of Neisseria bacteria / ROC I 280247 / USA 10/867,662 / European 04015231.6


  4. Apparatus for measuring HbAlc /Taiwan M383733.


申請中專利:

  • 鮑氏不動桿菌(Acinetobacter baumannii)多胜肽抗原及其抗體以及編碼該抗原之核酸


  • 榮  譽
       
    授課課程
     
    1. 分子生物學技術(一)、(二)
    2. 分子收物學 3. 免疫學導論
    4. 免疫學特論
    5. 文獻研討
    6. 專題討論
    7. 專題研究