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許文輝
教授Wen-Hwei Hsu BACK |
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美國密西根州立大學 博士
whhsu@dragon.nchu.edu.tw
辦公室電話: 04-22840485#237
實驗室電話: 04-22840485#238
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簡要經歷 |
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服務機關
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服務部門/系所 |
職稱
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起訖年月
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現職:國立中興大學
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分子生物學研究所 |
教授兼院長 |
2007/08~2010/07 |
經歷:國立中興大學
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分子生物學研究所
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教授兼所長 |
2004/
08至2007/ 07
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經歷:國立中興大學 |
分子生物學研究所
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教授兼所長 |
1995/
08至1998/ 07 |
經歷:中興大學
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分子生物學研究所
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教授
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1992/
04 ~ |
食品工業發展研究所
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菌種保存及研究中心 |
正研究員兼主任 |
1989/
05至1992/ 03 |
食品工業發展研究所 |
應用微生物組 |
正研究員兼組長
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1986/
07至1989/ 05 |
食品工業發展研究所
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食品科學組 |
研究員 |
1983/
03至1986/ 06 |
食品工業發展研究所
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食品微生物組 |
副研究員
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1972/
08至1983/ 03 |
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研究方向 |
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工業用特殊酵素基因的選殖、大量表現及人工演化是研發工作之主軸,
目的在於降低代謝產物、機能性胜?及原料藥之生產成本,終極目標是將衍生的技術轉移到產業界。現階段的研發方向是: |
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建立微生物基因表現平台,特別是針對 Aspergillus oryzae、Rhizopus
oryzae 及Corynebacterium glutamicum。
- 開發機能性及營養性胜?所需的特殊酵素,例如 leucine aminopeptidase,
carboxypeptidase 及 aspartate protease。
- 利用生物轉換法生產降血壓藥物之前驅物 homophenylalanine,特別是針
對 racemase、L-hydantoinase…等酵素之人工演化。
- 探索鬆弛支氣管及消除鼻黏膜腫脹藥物[(+)-pseudoephedrine及L-
phenylephrine]生合成之相關酵素,包括各種ketone reductases。
- 建構土壤微生物多元性基因庫(metagenomic library),選殖能分解
paclobutrazol之基因,經系列組合後,進行蓮霧園之復育。
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近期著作 |
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(A)
期刊論文 (★ , corresponding author)
- Chen, I. C., V. Thiruvengadam, W. D. Lin, H. H. Chang, and W. H. Hsu★. 2009.
Lysine racemase: a novel non-antibiotic selectable marker for plant transformation.
Plant Molecular Biology (In Press)
- Lo, H. H., W. D. Lin, S. K. Hsu, and W. H. Hsu★. 2009.
Asymmetrically simultaneous synthesis of L-Homophenylalanine and
N6-Protected-2-oxo-6-amino-hexanoic acid by engineered Escherichia coli
aspartate aminotransferase. Biotechnology Progress (In Press)
- Chen, C. C., Y. C. Cho, C. C. Lai, and W. H. Hsu★. 2009.
Purification and characterization of a new rhizopuspepsin from Rhizopus oryzae
NBRC 4749. Journal of Agricultural and Food Chemistry 57:6742-6747. (SCI)
- Chen, I. C., W. D. Lin, S. K. Hsu, V. Thiruvengadam, and W. H. Hsu★. 2009.
Isolation and characterization of a novel lysine racemase from a soil metagenomic library.
Applied and Environmental Microbiology 75:5161-5166. (SCI)
- Lo, C. K., C. H. Kao, W. C. Wang, H. M. W, W. H. Hsu, L. L. Lin, and H. Y. Hu. 2009.
Engineering of the critical residues at the stereochemistry-gate loops of Brevibacillus
agri dihydropyrimidinase for the production of L-Homophenylalanine. Process Biochemistry 44:309-315. (SCI)
- Chi, M. C., P. L. Ong, W. H. Hsu, Y. H. Chen, H. B. Huang, and L. L. Lin. 2008.
Role of the invariant Asn345 and Asn435 residues in a leucine aminopeptidase from Bacillus
kaustophilus as evaluated by site-directed mutagenesis. International Journal of
Biological Macromolecules 43:481-487. (SCI)
- Chen, C. L., T. Y. Pan, S. C. Kan, Y. C. Kuan, L. Y. Hong, K. R. Chiu, C. S. Sheu,
J. S. Yang, W. H. Hsu, and H. Y. Hu. 2008. Genome sequence of the lytic bacteriophage P1201
from Corynebacterium glutamicum NCHU 87078: evolutionary relationships to phages from Corynebacterineae.
Virology 378:226-232. (SCI)
- Chen, Y. J., W. C. Ku, L. T. Feng, M. L. Tsai, C. H. Hsieh, W. H. Hsu, W. F. Liaw, C. H. Hung,
and Y. J. Chen. 2008. Nitric oxide physiological responses and delivery mechanisms probed by water-soluble
Roussin's red ester and {Fe(NO)2}10 DNIC. Journal of American Chemical Society 130:10929-10938. (SCI)
- Hung, C. P., H. F. Lo, W. H. Hsu, S. C. Chen, and L. L. Lin. 2008.
Immobilization of Escherichia coli novablue gamma-glutamyltranspeptidase in
Ca-alginate-kappa-carrageenan beads. Applied Biochemistry and Biotechnology 150:157-170. (SCI)
- Kao, C. H., H. H. Lo, S. K. Hsu, and W. H. Hsu★. 2008.
A novel hydantoinase process using recombinant Escherichia coli cells with dihydropyrimidinase
and L-N-carbamoylase activities as biocatalyst for the production of L-homophenylalanine.
Journal of Biotechnology 134:231-239. (SCI)
- Ong, P. L., Y. F. Yao, Y. M. Weng, W. H. Hsu, and L. L. Lin. 2008.
Residues Arg114 and Arg337 are critical for the proper function of Escherichia coli gamma-glutamyltranspeptidase.
Biochemical and Biophysical Research Communications 366:294-300. (SCI)
- Hsu, S. K., H. H. Lo, W. D. Lin, I. C. Chen, C. H. Kao, and W. H. Hsu★. 2007.
Stereoselective synthesis of L-homophenylalanine using the carbamoylase method with in
situ racemization via N-acylamino acid racemase. Process Biochemistry 42:856-862. (SCI)
- Lee, Y. C., H. R. Chien, and W. H. Hsu★. 2007.
Production of N-acetyl-D-neuraminic acid by recombinant whole cells expressing Anabaena sp.
CH1 N-acetyl-D-glucosamine 2-epimerase and Escherichia coli N-acetyl-D-neuraminic acid lyase.
Journal of Biotechnology 129:453-460. (SCI)
- Lee, Y. C., H. M. Wu, Y. N. Chang, W. C. Wang, and W. H. Hsu★. 2007.
The central cavity from the (Alpha/Alpha)6 barrel structure of Anabaena sp.
CH1 N-acetyl-D-Glucosamine 2-epimerase contains two key histidines for reversible conversion.
Journal of Molecular Biology 367:895-908. (SCI)
- Lin, L. L., M. H. Chen, H. R. Chien, S. C. Kan, C. C. Chen, H. Y. Hu, and W. H. Hsu★. 2007.
Characterization of a bifunctional aminoacylase/carboxypeptidase from radioresistant bacterium
Deinococcus radiodurans R1. Journal of Biotechnology 128:322-334. (SCI)
- Hsu, S. K., H. H. Lo, C. H. Kao, D. S. Lee, and W. H. Hsu★. 2006.
Enantioselective synthesis of L-homophenylalanine by whole cells of recombinant
Escherichia coli expressing L-aminoacylase and N-acylamino acid racemase genes from Deinococcus radiodurans
BCRC12827. Biotechnology Progress 22:1578-1584. (SCI)
- Tsai, S. Y., S. C. Lin, S. Y. Suen, and W. H. Hsu. 2006.
Effect of number of poly(His) tags on the adsorption of engineered proteins on immobilized metal
affinity chromatography adsorbents. Process Biochemistry 41:2058-2067. (SCI)
- Lin, L. L., P. R. Chou, Y. W. Hua, and W. H. Hsu. 2006. Over-expression,
one-step purification, and biochemical characterization of a recombinant γ–glutamyltranspeptidase
from Bacillus licheniformis. Applied Microbiology and Biotechnology 73:103-112. (SCI)
- Chiu, W. C., J. Y. You, J. S. Liu, S. K. Hsu, W. H. Hsu, C. H. Shih, J. K. Hwang, and W. C. Wang. 2006.
Structure-stability-activity relationship in covalently cross-linked N-carbamoyl D-amino
acid amidohydrolase and N-acylamino acid racemase. Journal of Molecular Biology 359:741-753. (SCI)
- Chen, C. H., H. Y. Hu, Y. C. Cho, and W. H. Hsu. 2006. Screening of compactin-resistant
microorganisms capable of converting compactin to pravastatin.
Current Microbiology 53:108-112. (SCI)
- Lin, L. L., W. H. Hsu, W. Y. Hsu, S. C. Kan, and H. Y. Hu. 2005.
Phylogenetic analysis and biochemical characterization of a thermostable dihydropyrimidinases
from alkaliphilic Bacillus sp. TS-23. Antonie van Leeuwenhoek International Jouranal of General
and Molecular Microbiology 88:189-197. (SCI)
- Huang, H. B., M. C. Chi, W. H. Hsu, W. C. Liang, and L. L. Lin. 2005.
Construction and one-step purification of Bacillus kaustophilus leucine aminopeptidase fused
to the starch-binding domain of Bacillus sp. strain TS-23 α–amylase. Bioprocess Biosyst
Eng 27:389-398 (SCI)
- Chen, C. H., J. C. Cheng, Y. C. Cho, and W. H. Hsu★. 2005.
A gene cluster for the fatty acid catabolism from Pseudonocardia autotrophica BCRC12444.
Biochemical and Biophysical Research Communications 329:863-868. (SCI)
- Lo, H. H., S. K. Hsu, W. D. Lin, N. L. Chan, and W. H. Hsu★. 2005.
Asymmetrically synthesis of L-homophenylalanine using engineered Escherichia coli aspartate
aminotransferase. Biotechnology Progress 21:411-415. (SCI)
- Lo, H. F., Y. H. Chen, N. W. Hsiao, H. L. Chen, H. Y. Hu, W. H. Hsu, and L. L. Lin. 2005.
Stabilization of a truncated Bacillus sp. strain TS-23 α-amylase by replacing histidine-436 with aspartate.
World Journal of Microbiology & Biotechnology 21:411-416. (SCI)
- Suen, R. B., S. C. Lin, and W. H. Hsu. 2004. Hydroxyapatite-based immobilized
metal affinity adsorbents for protein purification. Journal of Chromatography A 1048:31-39. (SCI)
- Ho, L. F., S. Y. Li, S. C. Lin, and W. H. Hsu. 2004. Integrated enzyme purification and
immobilization processes with immobilized metal affinity adsorbents.
Process Biochemistry 39:1573-1581. (SCI)
- Chi, M. C., W. M. Chou, C. H. Wang, W. Chen, W. H. Hsu, and L. L. Lin. 2004.
Generating oxidation-resistant variants of Bacillus kaustophilus leucine aminopeptidase
by substitution of the critical methionine residues with leucine. Antonie van
Leeuwenhoek International Jouranal of General and Molecular Microbiology 86:355-362. (SCI)
- Chi, M. C., W. M. Chou, W. H. Hsu, and L. L. Lin. 2004.
Identification of amino acid essential for the catalytic reaction of
Bacillus kaustophilus leucine aminopeptidase.
Bioscience Biotechnology Biochemistry 68:1794-1797. (SCI)
- Wang, W. C., W. C. Chiu, S. K. Hsu, C. L. Wu, C. Y. Chen, J. S. Liu, and W. H. Hsu★. 2004.
Structural basis for catalytic racemization and substrates specificity of an N-Acylamino acid
racemase homologue from Deinococcus radiodurans. Journal of Molecular Biology 342:155-169. (SCI)
- Hua, Y. W., M. C. Chi, H. F. Lo, W. H. Hsu, and L. L. Lin. 2004.
Fusion of Bacillus stearothermophilus leucine aminopeptidase II with the raw
starch-binding domain of Bacillus sp. strain TS-23 α-amylase generates a chimeric enzyme
with enhanced thermostability and catalytic activity.
Journal of Industrial Microbiology & Biotechnology 31:273-277. (SCI)
- Hsu, S. K., L. L. Lin, H. H. Lo, and W. H. Hsu★. 2004.
Mutational analysis of feedback inhibition and catalytic sites of prephenate dehydratase from
Corynebacterium glutamicum. Archives of Microbiology 181:237-244. (SCI)
- Lin, L. L., W. H. Hsu, C. P. Wu, M. C. Chi, W. M. Chou, and H. Y. Hu. 2004.
A thermostable leucine aminopeptidase from Bacillus kaustophilus CCRC 11223.
Extremophiles 8:79-87. (SCI)
- Chang, C. T., H. F. Lo, M. C. Chi, C. Y. Yao, W. H. Hsu, and L. L. Lin. 2003.
Identification of essential histidine residues in a recombinant α-amylase of thermophilic
and alkaliphilic Bacillus sp. strain TS-23. Extremophiles 7:505-509. (SCI)
- Lo, H. H., C. H. Kao, D. S. Lee, T. K. Yang, and W. H. Hsu★. 2003.
Enantioselective synthesis of (S)-2-amino-4-phenylbutanoic acid by the hydantoinase method.
Chirality 15:699-702. (SCI)
- Chen, C. Y., W. C. Chiu, J. S. Liu, W. H. Hsu★, and W. C. Wang. 2003.
Structural basis for catalysis and substrate specificity of Agrobacterium radiobacter
N-carbamoyl-D-amino-acid amidohydrolase. Journal of Biological Chemistry 278:26194-26201. (SCI)
- Kao, C. H., and W. H. Hsu★. 2003. A gene cluster involved in pyrimidine reductive
catabolism from Brevibacillus agri NCHU1002. Biochemical and Biophysical
Research Communications 303:848-854. (SCI)
- Hu, H. Y. W. H. Hsu, and H. R. Chien. 2003.
Characterization and phylogenetic analysis of a thermostable N-carbamoyl-L-amino acid
amidohydrolase from Bacillus kaustophilus CCRC11223. Archives of Microbiology 179:250-257. (SCI)
- Lin, L. L., H. F. Lo, W. Y. Chiang, H. Y. Hu, W. H. Hsu, and C. T. Chang. 2003.
Replacement of methionine 208 in a truncated Bacillus sp. TS-23 α-amylase with oxidation-resistant
leucine enhances its resistance to hydrogen peroxide. Current Microbiology 46:211-216. (SCI)
- Chien, H. R., C. L. Hsu, H. Y. Hu, W. C. Wang, and W. H. Hsu★. 2002.
Enahncing oxidative resistance of Agrobacterium radiobacter N-carbamoyl D-amino acid
amidohydrolase by engineering solvent-accessible methionine residues. Biochemical and
Biophysical Research Communications 297:282-287. (SCI)
- Lo, H. F., L. L. Lin, W. Y. Chiang, M. C. Chie, W. H. Hsu, and C. T. Chang. 2002.
Deletion analysis of the C-terminal region of the α-amylase of Bacillus sp. Strain TS-23.
Archives of Microbiology 178:115-123. (SCI)
- Lin, L. L., H. F. Lo, J. N. Chen, K. L. Ku, and W. H. Hsu. 2002.
Isolation of a recombinant Bacillus sp. TS-23 α-amylase by adsorption-elution on raw starch.
Starch / Staerke 54:338-342. (SCI)
- Chien, H. C. L., L. Lin, S. H. Chao, C. C. Chen, W. C. Wang, C. Y. Shaw, Y. C. Chen,
H. Y. Hu, and W. H. Hsu★. 2002. Purification, characterization, and genetic analysis of a
leucine aminopeptidase from Aspergillus sojae. Biochimica et Biophysica Acta 1576:119-126. (SCI)
- Lo, H. F., L. L. Lin, C. C. Li, W. H. Hsu, and C. T. Chang. 2001.
The N-terminal signal sequence and the last 98 amino acids of Bacillus sp.
TS-23 α-amylase are not essential for its secretion in Escherichia coli.
Current Microbiology 43:170-175. (SCI)
- Wang, W. C., W. H. Hsu★, F. T. Chien, and C. Y. Chen. 2001.
Crystal structure and site-directed mutagenesis studies of N-carbamyl-D-amino-acid
amidohydrolase from Agrobacterium radiobacter reveals a homtetramer and insight into
a catalytic cleft. Journal of Molecular Biology 306:251-261. (SCI)
- Lin, L. L., H. F. Liao, H. R. Chien, and W. H. Hsu★. 2001.
Identification of essential cysteine residues in 3-deoxy-D-arabino-heptulosonate-7-phosphate
synthase from Corynebacterium glutamicum. Current Microbiology 42:426-431. (SCI)
- Liao, H. F., L. L. Lin, H. R. Chien, and W. H. Hsu★. 2001.
Serine 187 is a crucial residue for allosteric regulation of Corynebacterium
glutamicum 3-deoxy-D-arabino- heptulosonate-7-phosphate synthase.
FEMS Microbiology Letters 194:59-64. (SCI)
- Lo, H. F., L. L. Lin, H. L. Chen, W. H. Hsu, and C. T. Chang. 2001.
Enzymatic properties of a SDS-resistant Bacillus sp. TS-23 α-amylase produced by
recombinant Escherichia coli. Process Biochemistry 36:743-750. (SCI)
- Lin, L. L., H. R. Chien, W. C. Wang, T. S. Hwang, H. M. Fu, and W. H. Hsu★. 2000.
Expression of Trigonopsis variabilis D-amino acid oxidase gene in Escherichia coli and
characterization of its inactive mutants. Enzyme and Microbial Technology 27:482-491. (SCI)
- Ju, S. S., L. L. Lin, H. R. Chien, and W. H. Hsu★. 2000.
Substitution of the critical Methionine residues in Trigonopsis variabilis
D-amino acid oxidase with leucine enhances its resistance to hydrogen peroxide.
FEMS Microbiology Letters 186:215-219. (SCI)
- Hwang, T. S., H. M. Fu, L. L. Lin, and W. H. Hsu★. 2000.
High-level expression of Trigonopsis variabilis D-amino acid oxidase in
Escherichia coli using lactose as inducer. Biotechnology Letters 22:655-658. (SCI)
- Yin, B. D., Y. C. Chen, S. C. Lin, and W. H Hsu. 2000.
Production of D-amino acid precursors with permeabilized recombinant
Escherichia coli with D-hydantoinase activity. Process Biochemistry 35:915-921. (SCI)
- Ma, Y. J., L. L. Lin, H. Roger Chien, and W. H. Hsu★. 2000.
Efficient utilization of starch by a recombinant strain of Saccharomyces cerevisiae
producing glucoamylase and isoamylase. Biotechnology and Applied Biochemistry 31:55-59. (SCI)
(B) 專書
- Tzean, S. S., S. C. Chiu, J. L. Chen,
S. H. Hseu, H. H. Cin, G. Y. Liou, C.
C. Chen, and W. H. Hsu. 1994. Penicillium
and related telomorphs, Food Industry
Research and Development Institute, Taiwan.
楊秋忠、趙維良、廖啟成、黃山內、曾顯雄、許文輝.
2003. 台灣土壤微生物之收集應用. 中正基金會, 台灣.
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專 利 |
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類別
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專利名稱
Title of patent |
國別
Country
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專利號碼
Patent number |
發明人
Inventor |
專利權人
Patent
holder |
專利期間 |
1 |
生澱粉吸附溶析法回收及純澱粉枝切脢
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ROC |
070723
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許文輝等
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食品研究所
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1995/02/21~
2017/08/06 |
2 |
Bioconversion
of cephalosporin C to glutaryl-7-aminocephalosporanic
acid |
USA |
USP. 5,597,704
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許文輝等
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食品研究所 |
1997/01/28~
2017/01/27 |
3 |
Method
for Recovery and Purification of Isoamylase
by Adsorption on Raw Starch |
USA |
USP. 5,811,277
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許文輝等
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食品研究所 |
1998/09/22~
2018/09/21 |
4 |
生產苯丙胺酸之菌株及其用於發酵製備苯丙胺酸之方法 |
ROC |
099197
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許文輝等
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食品研究所 |
1998/11/11~
2014/12/21 |
5 |
Thermostable
D-hydantoinase and the application thereof
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USA |
USP, 6,403,357
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許文輝、高肇鴻 |
中興大學 |
2002/06/11~
2021/04/18 |
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榮 譽 |
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1.
國科會傑出研究獎 (2004年)
2. 台灣農業化學會學術榮譽獎 (2004年)
3. 經濟部科技計畫成果優良獎 (1994年)
4. 中華民國食品科技學會食品學術獎 (1985年)
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授課課程 |
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1. 生物催化、轉換及分解
2. 工業微生物
3. 專題討論
4. 專題研究
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