陳良築 教授 Liang-Jwu Chen                     BACK




美國伊利諾大學博士
ljchen@dragon.nchu.edu.tw
辦公室電話: 04-22840485#233
實驗室電話: 04-22840485#234

簡要經歷
 
國立中興大學 分子生物學研究所 教授 1997/08-迄今
國立中興大學 生命科學院 副院長 2013/08-迄今
國立中興大學 分子生物學研究所 所長 2007/08至2010/07
國立中興大學 生物科技發展中心 主任 2004/08至2005/04
國立中興大學 生物科技發展中心 教學組組長 2001/08至2004/07
國立中興大學 分子生物學研究所 所長 1998/08至2001/07
國立中興大學 分子生物學研究所 副教授 1991/08至1997/07
美國康乃爾大學 湯普遜植物研究中心 訪問學者 1994/09至1995/08
國立中興大學 遺傳工程中心 副教授 1989/08至1991/07
美國伊利諾大學 植物系 博士後研究員 1989/05至1989/08

研究方向
 
1. 水稻榖粒發育相關基因功能研究及其表現調控分析。

在基礎研究上:利用水稻突變體材料,配合生物資訊及各項基因體分析技術,探討水稻榖粒發育相關基因之功能,期望經由基因功能之了解,作為未來作物生產改進之用。

2. 高價醫藥工業用蛋白基因轉殖及其利用-植物分子農場研究。

在應用研究上 :利用基因轉殖技術,繼續篩選具經濟價值之重要蛋白基因,將之轉入植物(包括水稻、番茄、大豆)基因體,使植物能大量表現外源蛋白,成為蛋白生產工廠,並達到發展植物為分子農場之目標。


近期著作
 
A. 期刊論文

  1. Lo, S-F, Ho, T-HD., Liu, Y-L., Jiang M-J., Hsieh K-T., Chen,K-T, Yu,L-C., Lee,M-H., Chen,C-Y., Huang,T-P., Syu,Z-J., Jan,Y-J., Kojima,M., Sakakibara,H., Chen,L-J*, Yu,S-M. (2015) Fine tuning the Green Revolution by Regulating Gibberellin Degradation (ready to submitted)


  2. Kuan, Y-C., Thiruvengadam, V., Lin, J-S., Liu, J-H., Chen, T-J. Wu, H-M., Hsu, W-H., Chen,L-J*. and Wang, W-C*, Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic crops (ready to submitted)


  3. Lo, S-F; Fan, M-J; Hsing, Y-I; Chen, L-J; Chen, S; Wen, I-C; Liu, Y-L; Chen, K-T; Jiang, M-J; Lin, M-K; Rao, M-Y; Yu, L-C; Ho, T-HD; Yu, S-M (2015) Genetic resources offer efficient tools for rice functional genomics research. Plant, Cell & Environment DOI: 10.1111/pce.12632 (SCI) IF=6.960, PLANT SCIENCES= xxx%


  4. Wu, C-S., Chen D-Y., Chang, C-F., Li, M-J., Hung, K-Y., Chen L-J., Chen, P-W. (2014) The promoter and the 5’-untranslated region of rice metallothionein OsMT2b gene are capable of directing high-level gene expression in germinated rice embryos. Plant Cell Report 33:793-806 DOI 10.1007/s00299-013-1555-6 (SCI) IF=2.826, PLANT SCIENCES=24.8%


  5. Lin, D-G., Chou, S-Y., Wang, A. Z., Wang, I-W., Tseng, T-H., Lai, C-C., Chen, L-J. Wang, C-S. (2014) A Proteomic Study of Rice Cultivar TNG67 and Its Aroma Mutant SA0420. The Plant Sciences 214:20-28 http://dx.doi.org/10.1016/j.plantsci.2013.09.010 (SCI) IF=2.997, PLANT SCIENCES=17.8%


  6. Wang J-D., Lo S-F., Li Y-S., Chen P-J., Lin S-Y., Ho T-Y., Lin J-H. Chen L-J*.(2013) Ectopic expression of OsMADS45 activates the upstream genes Hd3a and RFT1 at an early development stage causing early flowering in rice. Botanical Studies 54:12 (SCI) IF=1.1, PLANT SCIENCES=56.8% (corresponding author)


  7. Kanjanaphachoat P., Wei B-Y., Lo S-F., Wang I-W., Wang C-S., Yu S-M., Yen M-L., Chiu S-H., Lai C-C. Chen, L-J*. (2012) Serotonin accumulation in transgenic rice by over-expressing tryptophan decarboxlyase results in dark brown phenotype and stunted growth. Plant Molecular Biology 78:525-543. (SCI) IF=4.1, PLANT SCIENCES=8.5% (corresponding author)


  8. Wu,YY, Chou,YR, Wang,CS, Tseng,TH, Chen,LJ*, Tzen,JTC. (2010) Different effects on triacylglycerol packaging to oil bodies in transgenic rice seeds by specifically eliminating one of their two oleosin isoforms. Plant Physiology and Biochemistry 48(2-3), 81-89. (SCI) IF=2.5, PLANT SCIENCES=22.3% (corresponding author)


  9. Lee,TT, Chang,CC, Juang,RS, Chen,RB, Yang,HY, Chu,LW, Wang,SR, Tseng,TH, Wang,CS, Chen,LJ*, Yu,B. (2010) Porcine lactoferrin expression in transgenic rice and its effects as a feed additive on early weaned piglets. Journal of Agricultural and Food Chemistry 58(8), 5166-5173. (SCI) IF=3.2, AGRICULTURE, MULTIDISCIPLINARY=3.6% (corresponding author)


  10. Lee, T-T., Huang, C-C., Chen, C-L., Chen, L-J. Yu, B. (2010) Flavonoid, phenol and polysaccharide contents of Echinacea purpurea L. and its immunostimulant capacity in vitro. International Journal of Environmental Science and Development 1: 5-9 (EI)


  11. Lo, K-L., Peng, S-F., Chen, L-J. and Lin, B-Y. (2009) Tandem organization of StarkB element (22.8 kb) in the maize B chromosome. Mol. Genetics and Genomics 282: 131-139 (SCI)


  12. Lo, K-L., Lin, Y-P., Chen, L-J. and Lin, B-Y. (2009) Isolation and characterization of new maize B sequences from a microdissected library. Plant Mol. Biol. Reporter 27: 350-354 (SCI)


  13. Lo, S-F., Yang, S-Y., Ku-Ting Chen, K-T., Hsing, Y-I., Zeevaart, J.A.D., Chen, L-J* and Yu, S-M (2008) A novel class of Gibberellin 2-Oxidases control semidwarfism, tillering and adventitious root development in rice. The Plant Cell 20: 2603-2618 (SCI)


  14. Hsing, Y-I., et. al., Chen, L-J. and Yu, S-M. (2007) A rice gene activation/knockout mutant resource for high throughput functional genomics. Plant Mol. Biol. 63: 351-364 (SCI)


  15. Lin, C-H., Liang, Y-J. and Chen, L-J*. (2005) A conserved inverted repeat from rice plastome functions as an intrinsic transcription terminator. Chin. Sci. Bull. 50:1669-1672. (SCI)


  16. Lin, C-H., Tzeng, C-C. and Chen, L-J*. (2005) A point-mutated psbA gene transfromed into tobacco chloroplast genome confers s-triazine herbicide tolerance in developing seedlings. Plant Prot. Bull. 47: 187-194


  17. Lee, T. T., Kao, Y-W., Chung, M-C., Wang, C-S., Chen, L-J. and Tzen, J.T.C. (2005) Specific expression of a sesame storage protein in rice bran. Journal of Cereal Sci. 41: 23-29. (SCI)


  18. Liao, R-M., Tseng, T-H., Chen, L-J. and Wang, C-S. (2004) Molecular cloning of rice H2A gene by differential display. J. Agri. Assoc. 5: 25-41


  19. Lin, C.-H., Chen, Y.-Y., Tzeng, C.-C., Tsay, H.-S. and Chen, L.-J.* (2003) Expression of a Bacillus thuringiensis crylC gene in plastid confers high insecticidal efficacy against tobacco cutworm-a Spodoptera insect. Bot. Bull. Acad. Sin.44: 199-210 (SCI)


  20. Lee, T.T., Wang, M.C., Ho, C.W., Chen, L-J, Su, R-C., Wang, C-C and Tzen, J.T.C. (2003) Enhanced methionine and cysteine levels in transgenic rice seeds by the accumulation of sesame 2S albumin. Biosci. Biotech. Biochem. 67: 1699-1705 (SCI)


  21. Lin, C.-H., Tzeng, C.-C. and Chen, L.-J.* (2003) Transformation of Bacillus thuringiensis toxins genes and its application in crop pest control. Plant Prot. Bull. New5: 137-158


  22. Lin, C.-H., Tzeng, C.-C., Kao, S.-S. and Chen, L.-J.* (2003) Insecticidal efficacy and phytopathogenic antagonism of the epiphytic Erwinia herbicola strains transformed with the Bacillus thuringiensis cry1Aa1 gene. J. Agri. Assoc. 4: 17-29


  23. Lin, C.-H., Chang, M.-L., Tzeng, C.-C. and Chen, L.-J.* (2002) Co-transformation of the synthetic cry1A(c) and wild-type cry1C genes encoding Bacillus thuringiensis endotoxins into tobacco and their bipartite expression effects on insecticidal efficacy. Plant Prot. Bull. 44: 209-232


  24. Tzeng, C.-C., Kao, S.-S., Chen, L.-J., Chak, K. F. and Hou, R. F (2002) Cloning of cryIAc gene in a Bacillus thuringiensis isolate from Taiwan. Plant Prot. Bull. 44: 185-208


  25. Lin, C.-H., Huang, W.-C., Tzeng, C.-C. and Chen, L.-J.* (2002) Insecticidal activity and plasmid retention of the epiphytic Erwinia herbicola strains transformed with the Bacillus thuringiensis cry1Aa1 gene. Plant Prot. Bull. 44: 21-36.


  26. Wu, L.S.H., Wang, L.-D., Chen, P.-W., Chen, L.-J.* and Tzen, J. T. C. (1998) Genomic cloning of 18 kDa oleosin and detection of triacylglycerols and oleosin isoforms in maturing rice and postgerminative seedlings. J. Biochem.123:386-391 (SCI)


  27. Chen, P.-W. and Chen, L.-J.* (1997) Isolation of a cDNA clone (Accession No. U57638) encoding a type 2 metallothionein-like protein from rice embryo. Plant Physiol. 114: 1568. (SCI)


  28. Chen, P.-W., Fang, L.-W., Lin, J.-W., Tsay, H.-S., Wu, H.-K., and Chen, L.-J.* (1997) Isolation of cDNA clones for genes that are specifically expressed during embryogenesis in Oryza sativa. Bot. Bull. Acad. Sin. 38: 13-20. (SCI)


  29. Chen, P.-W., Chow S.-H. and Chen, L.-J.* (1997) Nucleotide Sequence of a cDNA (Accession No. U76004) Encoding Rice Bowman-Birk Proteinase Inhibitor. Plant Physiol. 113: 663. (SCI)


  30. Cheng, Y.-S., Lin, C.-H. and Chen, L.-J.* (1997) Transcription and processing of the gene for spinach chloroplast threonine tRNA in a homologous in vitro system. Biochem. Biophy. Res. Comm. 233: 380-385. (SCI)


  31. Wu, C.-Y., Lin, C.-H. and Chen, L.-J.* (1997) Identification of the transcription start site for the spinach chloroplast serine tRNA gene. FEBS Lett. 418: 157-161. (SCI)


  32. Lin, C.-Y., Wang, C.-S., Wang, H.-L., Chen, L.-J.* and Tsay, H.-S. (1996) The application of biotechnology to agricultural production. J. Agric. Aso. China New Series 176:11-37.


  33. Yiu, J.-C., Tseng, M.-J. and Chen, L.-J.* (1996) Transfer of the insecticidal crystal protein gene of Bacillus thuringiensis into broccoli, cauliflower and Pak-Choi. J. Chinese Soc. Hort. Sci 42: 312-330.


  34. Chan, M.-T., Chen, L.-J. and Chang, H.-H. (1996) Expression of Bacillus thuringiensis (B.t.) insecticidal crystal protein gene in transgenic potato. Bot. Bull. Acad. Sin. 37:17-23. (SCI)


  35. Chen, P.-W., Chai, Y.-J., Wang, L.-D., Tzen, J. T. C., Tseng, M.-J. and Chen, L.-J.* (1996) Two embryo-specific cDNAs (Accession No. U43930 and U43931) encoding two oleosin isoforms on the surface of oil bodies from rice. Plant Physiol. 110: 714. (SCI)


  36. Chen, P.-W and Chen, L.-J.* (1996) A rice embryo-specific cDNA (Accession No. U57641) encoding a late embryogenesis abundant group 3 protein. Plant Physiol. 111:1353.


  37. Chen, L.-J.* (1995) Quantifying termination of transcription by spinach chloroplast RNA polymerase using supercoiled templates containing tandem copies of the thra terminator. Bot. Bull. Acad. Sin. 36:95-100. (SCI)


  38. Chen, L.-J.* Liang, Y.-J., Jeng, S.-T, Orozco, E. M., Gumport, R. I., Lin, C.-H. and Yang, M.-T. (1995) Transcription termination at the Escherichia coli thra terminator by spinach chloroplast RNA polymerase in vitro is influenced by downstream DNA sequences. Nucleic Acids Res. 23: 4690-4697. (SCI)

B. 專利Patents
   

類別

專利名稱
Title of patent
國別 Country
專利號碼 Patent number
發明人 Inventor
專利權人 Patent holder
專利期間
國科會計畫編號
A
於穀類植物榖粒外皮表現外源蛋白質之方法
ROC
專利證號I332029
李滋泰
陳良築*
曾志正
國立中興大學
Issued on 2010-10-21 till 2023-11-27
NSC-87-2611-B-005-004
NSC-88-2311-B-005-031
NSC-89-2311-B-005-015
A Gibberellin 2-oxidase genes control plant growth and architecture USA

專利證號United States Patent No. US8,034,992 B2 余淑美
羅舜芳
陳良築*

Academia Sinica Issued on 2011-10-11 94S-1502
NSC-96-2317-B-005-007
A
用於降血脂之重組大豆儲藏性蛋白及其製備方法與應用Recombinant soybean storage protein for lowering triglyceride level in blood and method for preparing the same and use thereof
ROC
專利證號I392734
許文輝
楊明德
陳良築
曾夢蛟
王強生
林金和
王雯靜
國立中興大學
Issued on 2013-04-11 till 2030-06-07
95農科-6.1.3.-糧-Z1(5)
96農科-1.1.1.-糧-Z1(1)
97農科-1.1.1.-糧-Z1(1)
A Method of controlling plant growth and architecture by controlling expression of gibberellin 2-oxidase USA

專利證號United States Patent No. US8,426,677 B2 余淑美
羅舜芳
陳良築*
賀端華

Academia Sinica Issued on 2013-04-23 94S-1502
NSC-96-2317-B-005-007
A Mutant gibberellin 2-oxidase genes and uses thereof USA

公開號
WO201408
5763 A1
余淑美
羅舜芳
陳良築*
賀端華

Academia Sinica announced on 2014-06-05 94S-1502
NSC-96-2317-B-005-007

C. 研討會論文
 


國外會議論文

  1. Lo,S-F, Hsing,Y-I., Chen,C-G., Chen,L-J, Ho, T-HD., Yu,S-M. (2014) Advanced rice functional genomics study using the Taiwan Rice Insertional Mutagenesis (TRIM) population. The 12th International Symposium on Rice Functional Genomics. Tucson, AZ, USA November 16-19, 2014.


  2. Hsieh K-T.,Lo,S-F, Hu, T-J., Wang, I-W., Yu,S-M. and Chen,L-J.* (2014) Differential expression and functional analysis of rice GA2-oxidase genes. Plant Biology 2014, Portland, Oregon USA. July 12- July 16, 2014. P20024-C.


  3. He, Hong-Yi, Ma, Wan-Chin, Lai, Yen-Hsun, Fung Kit-Man, Wang, I-Wen, Wu, Ming-Tse, Lai, Chian-Chen, Yang, Ming-Te and Chen, L-J.* (2013) Mass production of active peptides VVYP in transgenic rice and its applications as functional food in reducing blood lipids. Plant Biology 2013, Providence, RI USA. July 20- July 24, 2013. P06027


  4. Yu S-M., Lo S-F., Chen Y-S., Ho D. T-H. and Chen, L-J. (2013) Strategies for breeding rice for multiple stresses tolerant without yield penalty. Plant Biology 2013, Providence, RI USA. July 20- July 24, 2013. P01012


  5. Chen P-W., Wu C-S., Chang C-F., Li, M-J., Chen, D-Y., Hung K-Y. and Chen, L-J. (2013) Characterization of a strongly active rice metallothionein OsMT2b gene promoter in germinated rice seed and cell culture. Plant Biology 2013, Providence, RI USA. July 20- July 24, 2013. P06031


  6. Lo, S-F. Ho, T-H. Liu, Y-L. Jiang, M-J. Hsieh, K-T. Chen, L-J.*, and Yu, S-M. (2012) Control of Gibberellin Levels by Point-mutated Gibberellin 2-Oxidases Enhances Yield and Stress Tolerance in Rice. The 10th International Symposium on Rice Functional Genomics. Chiang Mai, Thailand, November 26-29, 2012. (PG-21)


  7. Wang, J-D, Lo S-F, Li, Y-S, Chen, P-J, Lin, J-H and Chen, L-J*. (2012) Ectopic expression of OsMADS45 activates the upstream genes Hd3a and RFT1 at an early development stage causing early flowering in rice. The 10th International Symposium on Rice Functional Genomics. Chiang Mai, Thailand, November 26-29, 2012. (PC-05)


  8. Lo S-F. , Li T-Y., Yang H-Y., He, H-Y., Liang, C., Yu S-M. and Chen, L-J.* (2012) Functional analysis of three activated genes 048-3, 048-4 and 048-7 in an early flowering T-DNA insertion rice mutant M52048. Plant Biology 2012, Austin, Texas USA. July 20- July 24, 2012. P06047


  9. Hsieh, K-T., Lo, S-F., Wang, I-W., Yu, S-M and Chen, L-J.* (2011) Functional study of a rice GA2-oxidase gene OsGA2ox2. The 9th International Symposium on Rice Functional Genomics. Taipei, Taiwan, November 07-09, 2011. (PB-13)


  10. Li, T-Y., Lo, S-F., Yang, H-Y., He, H-Y., Liang, C., Yu S-M and Chen, L-J.* (2011) Characterization of an early flowering T-DNA insertion rice mutant M52048. The 9th International Symposium on Rice Functional Genomics. Taipei, Taiwan, November 07-09, 2011. (PB-15)


  11. Lo, S-F., Hsing, Y-I.., Ho, T-H., Ku, SB., Quick, W.P, Mabilangan, A., Leung, H., Bruskiewich, R., Fan, M-J., Chen, L-J. and Yu, S-M. (2011) Screening of a T-DNA-Tagged gene activation/knockout rice mutant library for identification of genes controlling the C4 photosynthesis pathway. The 9th International Symposium on Rice Functional Genomics. Taipei, Taiwan, November 07-09, 2011. (PL-16)
  12. Kanjanaphachoat, P., Lo, S-F., Wang, I-W., Wang, C-S., Yu, S-M. and Chen, L-J.* (2010) Serotonin accumulation in transgenic rice by over-expression of tryptophan decarboxlyase results in dark brown phenotype and reduced fertility. Plant Biology 2010, Montreal, Canada. July 31- August 4, 2010. P08124


  13. Wu, Y-Y., Chen, P-W., Lee, C-F., Wang, I-W., Wang, C-S. and Chen, L-J.* (2010) Characterization of Osp40s and their effects on rice growth and development。 Plant Biology 2010, Montreal, Canada. July 31- August 4, 2010. P04073


  14. Lo, S-F., Yang, S-Y., Chen, K-T., Hsing, Y-I., Zeevaart, J.A.D., Yu, S-M. and Chen, L-J.* (2009) A Novel Class of Gibberellin 2-Oxidases Control semidwarfism, tillering and root development in rice。The 7th International Symposium on Rice Functional Genomics. Manila, Philippines, November 16-19, 2009. (P1-2)


  15. Lo, S-F., Hsing, Y-I., Chern, C-G., Chen, L-J.* and Yu, S-M. (2009) Rice functional genomics study using the Taiwan Rice Insertional Mutagenesis (TRIM) population。The 7th International Symposium on Rice Functional Genomics. Manila, Philippines, November 16-19, 2009. (P1-30)


  16. Lo, S-F., Tseng, T-H., Chern, C-G., Yu, S-M. and Chen, L-J.* (2009) Overexpression of a putative Flavonoid 3'- hydroxylase (Cytochrome P450 75B2) resulted in the reduction of grain weight and grain numbers in rice。The 9th International Society for Plant Molecular Biology. St. Louis, Missouri, USA, October 25-30, 2009 (#1938)


  17. Lo, S-F., Yu, S-M. and Chen, L-J.* (2008) Activation of a putative Flavonoid 3'- hydroxylase (Cytochrome P450 75B2) gene resulted in the reduction of grain weight and grain numbers per panicle in rice。 The 6th International Rice Functional Genomics Symposium. Jeju, South Korea, November 10-12, 2008.


  18. Lo, S-F., Yang, S-Y., Chen, K-T., Hsing, Y-I., Zeevaart, J.A.D., Yu, S-M. and Chen, L-J.* (2008) Functional analyses of three C20-classes of gibberellin 2-oxidases that regulate biosynthesis of GAs and control rice growth。The 7th Cross-Strait Symposium on Plant Molecular Biology and Biotechnology. Kunming, China, August 23-30, 2008.


  19. Lo, S-F., Yang, S-Y., Chen, K-T., Hsing, Y-I., Zeevaart, J.A.D., Yu, S-M. and Chen, L-J.* (2008) A Novel Class of Gibberellin 2-Oxidases Control Rice Growth and Architecture。Gordon Research Conference on Plant Molecular Biology. Holderness, NH, USA, July 13-18, 2008. (Invited Oral and Poster presentation)


國內會議論文

  1. Hsieh, K-T., Lo, S-F, Hu, T-J., Wang, I-W., Yu, S-M. and Chen, L-J.* (2014) Differential expression and functional analysis of rice GA2-oxidase genes. 2014 植物分子生物學研討會, 溪頭, Taiwan. July 04- July 06, 2014. PA10.


  2. Chang, F-H., Huang, J-F., Li, T-Y., Chen., Y-C., Lo, S-F, Hsie, K-T. and Chen,L-J.* (2014) Functional analysis of the aberrantly developed panicles in OsMADS34 and OsCP7 genes over-expressed transgenic rice. 2014 植物分子生物學研討會, 溪頭, Taiwan. July 04- July 06, 2014. PB29.


  3. Liao,C-C., Lo,S-F., Chen, L-J. and Chu, Y-W. (2014) Using machine learning approach to predict the flanking gene expression in T-DNA insertion rice mutants. 2014 植物分子生物學研討會, 溪頭, Taiwan. July 04- July 06, 2014. PB31


  4. Tsai, S-R, He, H-Y, Ma, W-Ch, Lai, Y-H, Lyu, S-Y, Wang, I-W, Zeng, C-S.,and Chen, L-J.* (2014) Breeding for nutritional enhanced elite rice variety containing active peptides VVYP. 2014 植物分子生物學研討會, 溪頭, Taiwan. July 04- July 06, 2014. PB30


  5. Chen, L-J.* (2012) Functional study of rice genes using T-DNA insertion mutants。「轉譯農學分子生物論壇」。 嘉義大學生物農業科技系。2012 年10月11日(Invited speaker)


  6. He, H-Y., Ma, Ali, Lee, T-T., Wang,- I-W., Lin,G-S., Cheng, L-C., Yang, M-T., Hsu, W-H., Yu, B., Wu, M-T. and Chen L-J. * (2012) Lactoferrin and active peptides expression in transgenic rice and their uses as feed additives or nutritionally-enhanced foods. 2012 台灣生物科技產學新知研討會National Cheng-Kung University, Tainan, Taiwan, May 30, 2012


  7. Kanjanaphachoat, P., Lo, S-F., Wang, I-W., Wang, C-S., Yu, S-M. and Chen, L-J.* (2011) Serotonin accumulation in transgenic rice by over-expression of tryptophan decarboxlyase results in dark brown phenotype and stunted growth. 第九屆海峽兩岸植物分子生物學及生物技術學術研討會Taichung, Taiwan. January 18-19/2011


  8. Chen, L-J.* (2010) 生物科技新知--植物功能性基因體分析。「精進農業生物技術研習班」。 國立臺彎大學園藝學系暨生物科技研究所。2010 年09月25日(Invited speaker)


  9. Chen, L-J.* (2010) 利用轉基因水稻生產營養強化食品及作為分子農場之探討。 「2010轉譯農學暨台灣農業生物技術研討會」。 國立中興大學農資學院、社管院、生物科技發展中心。 臺中,2010 年09月02日 (Invited speaker)


  10. Chen, L-J.* (2009) Expression of porcine lactoferrin and active peptides VVYP in transgenic rice. 營養強化基因改造食品與複製動物食品安全評估研討會。 行政院衛生署食品衛生處,臺北,2009 年8月6日 (Invited speaker)


  11. Cheng C-C, Lin C-C, Li A-Y, Huang W-H, Wang I-W, Chen J. W., Chen, L-J.* (2008) Expression of rice auxin response factor 10 (ARF10) is down-regulated by osa-miR160a. The16th Symposium on Recent Advances in Cellular and Molecular Biology, Ken Ting, Taiwan January, 22-25, 2008 (Poster Award)
  12. Huang, W-H., Lin, C-C, Li, A-Y, , Cheng, C-C, Wang, I-W., Chen J. W., Chen, L-J.* (2008) Over-expression of osa-miR167b inhibits the expression of auxin response factor 6b that affects shoot growth in rice. The16th Symposium on Recent Advances in Cellular and Molecular Biology, Ken Ting, Taiwan January, 22-25, 2008


  13. An-Ye Li, Lin, C-C, Huang, W-H., Cheng, C-C, Wang, I-W., Chen J. W., Chen, L-J.* (2008) Over-expression of osa-miR529 inhibits the expression of REH1 that resulted in a dwarf and tiller number increasing phenotype in rice. The16th Symposium on Recent Advances in Cellular and Molecular Biology, Ken Ting, Taiwan January, 22-25, 2008

D. 技術報告及其他 Sequences submitted to GenBank
 
  1. Lin, C.H., and Chen, L.J. (2003) Chloroplast transformation vector pN-IC101. Accession number AY442171


  2. Liao, R. M., Tseng, T. H., Chen, L. J. and Wang, C. S. (1999) Cloning of rice early embryogenesis related gene H2A by using differential display. Accession number AF193345.


  3. Lou, H-R., Chen, P-W. and Chen, L-J. (1998) A genomic DNA sequence for an unknown gene Ose731.
    Accession number AF050155.


  4. Chen, L-J. and Chen, P-W. (1998) A rice embryo-specific cDNA clone Ose731, complete cds.
    Accession number AF049892.


  5. Chen, P-W. and Chen, L-J. (1998) A rice embryo-specific cDNA clone Ose705, complete cds.
    Accession number AF049348.


  6. Chen, P-W. and Chen, L-J. (1998) A genomic DNA sequence coding for a rice metallothionein gene.
    Accession number AF048750.


  7. Chen, L-J., Wang, L-D. and Chen, P-W. (1998) A genomic DNA sequence coding for a rice group 3 LEA protein gene. Accession number AF046884.


  8. Wang, L-D. and Chen, L-J. (1997) Oryza sativa 18 kDa oleosin genomic DNA sequence.
    Accession number AF019212.


  9. Chen, L-J.and Chen, P-W. (1996) A Bowman-Birk type rice proteinase inhibitor.
    Accession number U76004.


  10. Chen, L-J. and Chen, P-W. (1996) Rice Ose730 cDNA clone, LEA-like gene, complete cds.
    Accession number U57641.


  11. Chen, L-J. and Chen, P-W. (1996) A Bowman-Birk type rice bran trypsin inhibitor.
    Accession number U57640.


  12. Chen, L-J., Chai, Y-J. and Chen, P-W. (1996) A rice embryo-specific gene with high homologous to a soybean GmPM3 gene, a hydrophobic LEA protein gene. Accession number U57639.
    Accession number U57639.


  13. Chen, L-J. and Chen, P-W. (1996) Rice Ose712 cDNA clone, Metallothionein-like gene, complete cds. Accession number U57638.


  14. Chen, L-J. and Chen, P-W. (1996) Rice Ose710 cDNA clone, Globulin-like gene, partial.
    Accession number U45322.


  15. Chen, L-J., Chai, Y-J. and Chen, P-W. (1996) Rice Ose709 cDNA clone, Globulin-like gene, partial.
    Accession number U45321.


  16. Chen, L-J., Lin, J-W. and Chen, P-W. (1996) Rice Ose704 cDNA clone, Globulin-like gene, partial.
    Accession number U45320.


  17. Chen, L-J., Wang, L-D. and Chen, P-W. (1996) Rice Ose703 cDNA clone, Globulin-like gene, partial.
    Accession number U45319.


  18. Chen, L-J., Chen, P-W., Chai, Y-J and Wang, L-D (1995) Rice 18 kDa oleosin cDNA, complete cds.
    Accession number U43931.


  19. Chen, L-J., Chen, P-W., Chai, Y-J and Wang, L-D (1995) Rice 16 kDa oleosin cDNA, complete cds.
    Accession number U43930.
E. 專書及特刊:
 
  1. 林志輝、鄭榮宗、陳良築 (2004)。葉綠體基因轉殖:第九章:pp 111-120。教育部顧問室生物技術科技教育改進計畫「植物基因轉殖之原理及應用」教材。 植物生物技術教學資源中心-國立中興大學生物科技發展中心主編。


  2. 陳良築、林志輝 (2004)。菸草-葉綠體轉殖技術:第一部分:基因轉殖技術,第一章:茄科植物,第一節:pp 9-16。教育部顧問室生物技術科技教育改進計畫「植物基因轉殖與分子檢測技術」教材。 植物生物技術教學資源中心-國立中興大學生物科技發展中心主編。


  3. 陳良築 吳妤憶 (2004)。南方轉漬法:第二部分:分子檢測技術,第三章:轉基因植物之分子檢測技術,第三節:pp 247-252。教育部顧問室生物技術科技教育改進計畫「植物基因轉殖與分子檢測技術」教材。 植物生物技術教學資源中心-國立中興大學生物科技發展中心主編。
計畫



  1. 科技部,水稻GA 2-oxidase基因家族成員之生理功能分析及其在耐逆境及增產上之應用,2015/08 ~ 2018/07。


  2. 農委會,創新與開發春化基因轉殖之觀賞花卉-創新與開發春化基因轉殖之蝴蝶蘭,2015/01 ~ 2015/12。


  3. 農委會,創新與開發春化基因轉殖之觀賞花卉-創新與開發春化基因轉殖之蝴蝶蘭,2014/01 ~ 2014/12。


  4. 農委會,迷你觀賞花卉之創新與開發-建立基因轉殖迷你及早開花之花卉的技術平台,2013/01 ~ 2013/12。


  5. 國科會,水稻GA 2-oxidase基因家族成員之生理功能及其基因轉錄調節機制之探討,2012/08 ~ 2015/07。


  6. 國科會,含活性胜肽的營養強化稻米之VVYP釋放量定量、生物活性分析及水稻品種育成,2012/08 ~2013/12。


  7. 農委會,迷你觀賞花卉之創新與開發-建立基因轉殖迷你及早開花之花卉的技術平台,2012/01 ~ 2012/12。


  8. 農委會,迷你觀賞花卉之創新與開發-建立基因轉殖迷你及早開花之花卉的技術平台,2011/01 ~ 2011/12。


  9. 國科會,含活性胜肽的營養強化稻米之VVYP釋放量定量、生物活性分析及水稻品種育成,2010/08 ~2012/07。


  10. 農委會,迷你觀賞花卉之創新與開發-建立基因轉殖迷你及早開花之花卉的技術平台,2010/01 ~ 2010/12。


  11. 國科會,利用T-DNA插入突變株之水稻基因功能研究—生長與榖粒發育異常突變株M52048及M48000A之特性及基因表現分析,2009/08 ~ 2012/07。


  12. 農委會,作物基因轉移技術之開發應用-開發基因轉殖大豆做為生物反應器─含降血脂機能性胜Gy1蛋白基因之轉殖及利用microRNA抑制基因表現技術之開發,2009/01~ 2009/12。


  13. 農委會,作物基因轉移技術之開發應用-開發基因轉殖大豆做為生物反應器─含降血脂機能性胜Gy1蛋白基因之轉殖及利用microRNA抑制基因表現技術之開發,2008/04/08~2008/12/31


  14. 國科會,利用水稻T-DNA插入突變系進行發育與抗逆境功能性基因體研究-利用水稻T-DNA插入突變系進行發育與抗逆境功能性基因體研究:水稻榖粒發育相關基因篩選與應用(2/2),2008/01/01~2008/12/31

專  利
 

類別

專利名稱
Title of patent
國別 Country
專利號碼 Patent number
發明人 Inventor
專利權人 Patent holder
專利期間
國科會計畫編號
A
於穀類植物榖粒外皮表現外源蛋白質之方法
ROC
專利證號I332029
李滋泰
陳良築*
曾志正
國立中興大學
Issued on 2010-10-21 till 2023-11-27
NSC-87-2611-B-005-004
NSC-88-2311-B-005-031
NSC-89-2311-B-005-015
A Gibberellin 2-oxidase genes control plant growth and architecture USA

專利證號United States Patent No. US8,034,992 B2 余淑美
羅舜芳
陳良築*

Academia Sinica Issued on 2011-10-11 94S-1502
NSC-96-2317-B-005-007
A
用於降血脂之重組大豆儲藏性蛋白及其製備方法與應用Recombinant soybean storage protein for lowering triglyceride level in blood and method for preparing the same and use thereof
ROC
專利證號I392734
許文輝
楊明德
陳良築
曾夢蛟
王強生
林金和
王雯靜
國立中興大學
Issued on 2013-04-11 till 2030-06-07
95農科-6.1.3.-糧-Z1(5)
96農科-1.1.1.-糧-Z1(1)
97農科-1.1.1.-糧-Z1(1)
A Method of controlling plant growth and architecture by controlling expression of gibberellin 2-oxidase USA

專利證號United States Patent No. US8,426,677 B2 余淑美
羅舜芳
陳良築*
賀端華

Academia Sinica Issued on 2013-04-23 94S-1502
NSC-96-2317-B-005-007
A Mutant gibberellin 2-oxidase genes and uses thereof USA

公開號
WO201408
5763 A1
余淑美
羅舜芳
陳良築*
賀端華

Academia Sinica announced on 2014-06-05 94S-1502
NSC-96-2317-B-005-007

榮  譽

  1. 2011,國立中興大學,產學合作績優獎。


  2. 2009,國科會,國科會大專學生參與專題研究計畫研究創作獎。


  3. 2009,經濟部工業局,【生物技術研發成果創意應用競賽】植物生技領域銅牌獎。


  4. 2007,國立中興大學,建教合作計畫績優獎。


  5. 2005,國立中興大學,建教合作計畫績優獎。


授課課程
 
1. 分子生物學

2. 高等分子生物學

3. 分子生物學技術

4. 植物基因調控

5. 作物分子農場之研發及應用

6. 文獻研討

7. 專題討論

8. 植物分子遺傳新知探討