簡要經歷　　研究方向　　近期著作　　專　　利　　榮　　譽　　授課課程

陳良築 教授
　Liang-Jwu Chen

美國伊利諾大學博士

ljchen@dragon.nchu.edu.tw

辦公室電話: 04-22840485#233
實驗室電話: 04-22840485#234

簡要經歷　　回頁首

研究方向　　回頁首

一、最近參與的研究計劃案：
1. 含豬乳鐵蛋白基因轉殖水稻之育成研究; 農委會; 2002/01/01 –迄今
2. 醫藥工業用酵素DHT, NCA及抗奈瑟氏腦膜炎雙球菌抗體基因之水稻轉殖; 農委會;
　2002/01/01 –迄今
3. 生產豬乳鐵蛋白轉殖水稻之隔離田間及安全性評估試驗; 農業國家型計畫; 2003/01/01 　
　–2004/12/31
4. 利用水稻T-DNA插入突變系進行發育與抗逆境功能性基因體研究: 水稻榖粒發育相關
　基因篩選與應用; 農業國家型計畫; 2004/01/01 –迄今
5. 葉綠體基因轉錄體研究: rpoZ角色之探討; 國科會; 2002/08/01 –2004/07/31
6. 應用Erwinia屬植物表生菌轉殖蘇力菌殺蟲基因開發植物保護新藥劑; 農業國家型計
　畫; 2004/01/01 –2004/12/31

二、研究主題：
1. 水稻榖粒發育相關基因功能研究及其表現調控分析。
2. 高價醫藥工業用蛋白基因轉殖及其利用-植物分子農場研究。

三、研究概況：
　　近年來以水稻為材料，研究水稻胚及穀粒發育相關基因之表現與選殖，累積了一些成果(Chen et al., 1996a,b; Chen et al., 1997a,b,c; Wu et al., 1998)。在水稻榖粒發育相關基因功能之分析研究方面，目前已篩得與水稻榖粒稔實、外型特異及發育異常之水稻突變株近50株，並已解讀其中27株之T-DNA插入點，及分析插入基因或鄰近基因表現之差異。這些水稻突變體，將成為未來研究水稻榖粒發育相關基因之重要材料。另外，本研究室過去篩得與水稻胚發育時期特有表現之基因有38個，大部分仍不知其功能，亦將利用RNAi之技術或誘導產生基因突變之方法，探討基因之功能。
　　而於水稻基因轉殖及應用方面，已有成功轉殖豬乳鐵蛋白基因之轉殖水稻現正進行隔離田間試驗中。另外也成功轉殖多個醫藥工業用酵素如D-hydantoinase, N-carbamoyl D-amino acid amidohydrolase及L-aminoacylase基因之轉殖水稻及芝麻2S貯藏性蛋白基因之轉殖水稻 (Lee et al., 2003; Lee et al., 2004)。

四、目前執行計劃
1. 利用水稻T-DNA插入突變系進行發育與抗逆境功能性基因體研究: 水稻榖粒發育相關
　基因篩選與應用; 農業國家型計畫; 2004/01/01 –迄今
2. 含豬乳鐵蛋白基因轉殖水稻之育成研究; 農委會; 2002/01/01 –迄今
3. 醫藥工業用酵素DHT, NCA及抗奈瑟氏腦膜炎雙球菌抗體基因之水稻轉殖; 農委會;
　2002/01/01 –迄今

五、未來研究重點
　　在基礎研究上：利用水稻突變體材料，配合生物資訊及各項基因體分析技術，探討水稻榖粒發育相關基因之功能，期望經由基因功能之了解，作為未來作物生產改進之用。
　　在應用研究上 ：利用基因轉殖技術，繼續篩選具經濟價值之重要蛋白基因，將之轉入植物(包括水稻、番茄、大豆)基因體，使植物能大量表現外源蛋白，成為蛋白生產工廠，並達到發展植物為分子農場之目標。

近期著作　　回頁首

A. 期刊論文
1. Lin, C.-H., Huang, W.-C., Tzeng, C.-C. and Chen, L.-J.* (2002) Insecticidal activity and plasmid retention of the epiphytic Erwinia herbicola strains transformed with the Bacillus thuringiensis cry1Aa1 gene. Plant Prot. Bull. 44: 21-36.

2. Tzeng, C.-C., Kao, S.-S., Chen, L.-J., Chak, K. F. and Hou, R. F (2002) Cloning of cryIAc gene in a Bacillus thuringiensis isolate from Taiwan. Plant Prot. Bull. 44: 185-208

3. Lin, C.-H., Chang, M.-L., Tzeng, C.-C. and Chen, L.-J.* (2002) Co-transformation of the synthetic cry1A(c) and wild-type cry1C genes encoding Bacillus thuringiensis endotoxins into tobacco and their bipartite expression effects on insecticidal efficacy. Plant Prot. Bull. 44: 209-232

4. Lin, C.-H., Tzeng, C.-C. and Chen, L.-J.* (2003) Transformation of Bacillus thuringiensis toxins genes and its application in crop pest control. Plant Prot. Bull. 45: 137-158

5. Lin, C.-H., Tzeng, C.-C., Kao, S.-S. and Chen, L.-J.* (2003) Insecticidal efficacy and phytopathogenic antagonism of the epiphytic Erwinia herbicola strains transformed with the Bacillus thuringiensis cry1Aa1 gene. J. Agri. Assoc. 4: 17-29

6. Lin, C.-H., Chen, Y.-Y., Tzeng, C.-C., Tsay, H.-S. and Chen, L.-J. * (2003) Expression of a wild-type Bacillus thuringiensis toxin gene in tobacco chloroplasts confers high efficacy of insect resistance. Bot. Bull. Acad. Sin. 44: 199-210

7. Lee, T.T., Wang, M.C., Ho, C.W., Chen, L-J, Su, R-C., Wang, C-C and Tzen, J.T.C. (2003) Enhanced methionine and cysteine levels in transgenic rice seeds by the accumulation of sesame 2S albumin. Biosci. Biotech. Biochem. 67: 1699-1705

8. Liao, R-M., Tseng, T-H., Chen, L-J. and Wang, C-S. (2004) Molecular cloning of rice H2A gene by differential display. J. Agri. Assoc. 5: 25-41

9. Lin, C-H., Liang, Y-J. and Chen, L-J.* (2005) A conserved inverted repeat from rice plastome functions as an intrinsic transcription terminator.  (Submitted)

10. Hsing, Y-I., et al., Chen, L-J. and Yu, S-M. (2005) High throughput generation and analysis of a rice gene activation/knockout library for functional genomics. (Submitted)

11. Lee, T. T., Kao, Y-W., Chung, M-C., Wang, C-S., Chen, L-J. and Tzen, J.T.C. (2005) Specific expression of a sesame storage protein in rice bran. J. Cereal Sci. 41: 23-29

12. Hsing, Y. I., et. al., L. J. Chen, and Yu, S-M. (2007) High throughput generation and analysis of a rice gene activation/knockout library for functional genomics. Plant Mol. Biol. 63:351-364.

B. 研討會論文

1. Chen, L-J., et. al. (2004) Plants as protein factories: plant-based production of biopharmaceuticals. 2004生物科技新知研討會，中興大學8月19-20日，2004, pp. 51-60

2. Chang, J-C., Yang, H-Y., Chen, R-B., Tsay, J-Y., Wang, C-S., Wang, S-R. and Chen, L-J. (2003) Production of porcine lactoferrin in transgenic rice. Internatioanl Symposium on Plant Biotechnology，I-Lan, Taiwan 10月 1-4日，2003. pp.72

3. Chen, H-C., Lee, K-C., Yang, H-Y., Kao, C-H., Hsu, W-H., Wang, C-S. and Chen, L-J. (2003) Expression and characterization of a thermostable D-hydantoinase gene in transgenic rice. Internatioanl Symposium on Plant Biotechnology，I-Lan, Taiwan 10月 1-4日，2003. pp.71

4. Lin, C-H., Tzeng, C-C. and Chen, L-J., (2003) Transformation of Bacillus thuringiensis toxin genes and its application in crop pest control. 植物保護管理永續發展研討會，臺中市科學博物館 9月 4日，2003. pp. 137-158.

5. Chen, L-J., et. al. (2003) Plant factories for protein productions. 2003生物科技新知研討會，中興大學8月 25日，2003

6. Tsay, J-Y., Chen, R-B., Chang J-C., Liao, C-H., Yang, H-Y., Wang, S-R. and Chen, L-J. (2002) 利用水稻生產豬乳鐵蛋白之研究。農業生物技術應用研討會，臺中市科學博物館 7月 10日，2002。 pp. 18

7. Chen, Y-J., Sun, P-K., Wang, L-D., Wu, Y-Y., Huang, Y-C., Chen, P-W., Wang, C-S. and Chen, L-J. (2002) Moleculare cloning of a rice LEA-like genesand its promoter activity and functional study in transgenic ric. 植物分子遺傳研討會，臺南曾文水庫 8月 30日 2002. pp. 21

8. Chen, L-J. et. al. (2002) 植物生物工廠。生物科技新知研討會，中興大學11月 11日，2002.

9. Tsay, J-Y., Chen, R-B., Chang J-C., Liao, C-H., Yang, H-Y., Wang, S-R. and Chen, L-J. (2001) Production of porcine lactoferrin in transgenic rice. The 3^rd Cross-strait Symposium on Plant Molecular Biology and Biotechnology, Hong Kong, August 5-11, 2001. pp.16

10. Lee, K.-C., Kao, C-H., Hsu, W.-H. and Chen, L.-J. (2001) Expression of a thermostable D-hydantoinase gene in transgenic rice plants. Plant Physiol. Suppl., 126-

11. Chen, Y.-J., Wang, L.-D., Chen, P.-W., Chung, M.-C. and Chen, L.-J. (2000) Molecular cloning of a LEA-like gene and characterization of its promoter activity and antisense gene effect in transgenic rice plants. Plant Physiol. Suppl., 123-

12. Lin, C.-H., Chang, M.-L., Chen, Y.-Y., Tzeng, C.-C., Tsay, H.-S. and Chen, L.-J. (1999) Expression of a wild-type Bacillus thuringiensis toxin gene in tobacco chloroplasts confers high efficacy of insect resistance. Plant Physiol. suppl., 120 -.

13. Wang, L.-D., Chen, P.-W., Chung, M.-C. and Chen, L.-J. (1998) Structure and expression analysis of a grioup 3 LEA protein from rice embryo. Plant Physiol. suppl., 117 -

14. Chen, L.-J. (1998) Crop improvement through transgenic approaches: application of Bacillus thuringiensis toxin and rice oleosin genes. In Abstracts of the 3rd Asia Crop Science Conference

15. Chen, P.-W. and Chen, L.-J. (1997) Isolation of cDNA clones for genes that are specifically expressed in the rice embryo and characterization of a rice metallothionein-like gene. In Abstracts of the 5th International Congress of Plant Molecular Biology :#372

16. Jeng, Y.-S., Lin, C.-H. and Chen, L.-J. (1997) Transcription and processing of spinach chloroplast threonine tRNA in a homologous in vitro system. Plant Physiol. suppl., 114: 248.

17. Chen, P.-W., Chai, Y.-J., Wang, L.-D., Chow, S.-H., Tseng, M.-J., Lin, J.-W., Tsay, H.-S., Wu, H.-K., and Chen, L.-J. (1996) Isolation of cDNA clones for genes that are specifically expressed in the rice embryo. In Abstracts of the 5th International Symposium on Rice Molecular Biology. #3-10

18. Chen, L-J. Liang, Y-J Lin, C.-H., and Jeng, S.-T. (1994) Transcription termination of the thra terminator by spinach chloroplast RNA polymerase. In Abstracts of APSB, The First International Symposium and Workshop.

19. Chen, L-J. and Liang, Y-J (1994) Transcription termination of the E.coli thra terminator by spinach chloroplast RNA polymerase is modulated by a not-yet-transcribed DNA sequence. In Abstracts of the 4th International Congress of Plant Molecular Biology :#329

20. Chen, P-W., Tzeng, C-C., Kao, S-S., Tsay, H-S. and Chen, L-J. (1993) Breeding for insect tolerant transgenic plants by using insecticidal crystal protein genes from Bacillus thuringiensis. The 7th International Congress of SABRAO 1993

21. Chen, P-W., Tzeng, C-C., Kao, S-S., Tsay, H-S. and Chen, L-J. (1993) Isolation and application of insecticidal crystal protein genes from Bacillus thuringiensis. In Proceeding for Plant Tissue and Cell Culture 1993. Taichung, Taiwan. pp 5-26.

22. Chang, T.-L. and Chen, L.-J. (1992). The divergently transcribed rbcL and atpB genes of rice plastid DNA are overlapped. Plant Physiol. suppl., 99: 91

C. 技術報告及其他

1. Chen, L-J., Chen, P-W., Chai, Y-J and Wang, L-D (1995) Rice 16 kDa oleosin cDNA, complete cds. Accession number U43930.

2. Chen, L-J., Chen, P-W., Chai, Y-J and Wang, L-D (1995) Rice 18 kDa oleosin cDNA, complete cds. Accession number U43931.

3. Chen, L-J., Wang, L-D. and Chen, P-W. (1996) Rice Ose703 cDNA clone, Globulin-like gene, partial. Accession number U45319.

4. Chen, L-J., Lin, J-W. and Chen, P-W. (1996) Rice Ose704 cDNA clone, Globulin-like gene, partial. Accession number U45320.

5. Chen, L-J., Chai, Y-J. and Chen, P-W. (1996) Rice Ose709 cDNA clone, Globulin-like gene, partial. Accession number U45321.

6. Chen, L-J. and Chen, P-W. (1996) Rice Ose710 cDNA clone, Globulin-like gene, partial. Accession number U45322.

7. Chen, L-J. and Chen, P-W. (1996) Rice Ose712 cDNA clone, Metallothionein-like gene, complete cds. Accession number U57638.

8. Chen, L-J., Chai, Y-J. and Chen, P-W. (1996) A rice embryo-specific gene with high homologous to a soybean GmPM3 gene, a hydrophobic LEA protein gene. Accession number U57639.Accession number U57639.

9. Chen, L-J. and Chen, P-W. (1996) A Bowman-Birk type rice bran trypsin inhibitor. Accession number U57640.

10. Chen, L-J. and Chen, P-W. (1996) Rice Ose730 cDNA clone, LEA-like gene, complete cds. Accession number U57641.

11. Chen, L-J.and Chen, P-W. (1996) A Bowman-Birk type rice proteinase inhibitor. Accession number U76004.

12. Wang, L-D. and Chen, L-J. (1997) Oryza sativa 18 kDa oleosin genomic DNA sequence. Accession number AF019212.

13. Chen, L-J., Wang, L-D. and Chen, P-W. (1998) A genomic DNA sequence coding for a rice group 3 LEA protein gene. Accession number AF046884.

14. Chen, P-W. and Chen, L-J. (1998) A genomic DNA sequence coding for a rice metallothionein gene. Accession number AF048750.

15. Chen, P-W. and Chen, L-J. (1998) A rice embryo-specific cDNA clone Ose705, complete cds. Accession number AF049348.

16. Chen, L-J. and Chen, P-W. (1998) A rice embryo-specific cDNA clone Ose731, complete cds. Accession number AF049892.

17. Lou, H-R., Chen, P-W. and Chen, L-J. (1998) A genomic DNA sequence for an unknown gene Ose731. Accession number AF050155.

18. Liao, R. M., Tseng, T. H., Chen, L. J. and Wang, C. S. (1999) Cloning of rice early embryogenesis related gene H2A by using differential display. Accession No. AF193345.

19.Lin, C.H., and Chen, L.J. (2003) Chloroplast transformation vector pN-IC101. Accession number AY442171

專　　利　　回頁首

榮　　譽　　回頁首

授課課程　　回頁首

1. 分子生物學(二)
2. 分子生物學技術(一)、(二)
3. 細胞分子生物學
4. 植物基因調控(一)、(二)
5. 文獻研討
6. 專題討論
7. 專題研究