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Education:
Employer:
2000 - Graduate Institute of Biotechnology, National Chung Hsing University (Taiwan)
Research interest: My research interest has been focused on elucidating the mechanism of action and the structure-function relationship of proteins. One ongoing project is to delineate the structural organization of the putative replicase encoded by the ORF 1 of bamboo mosaic virus. This protein has molecular mass of 155 KD, and has been proposed to be involved in the replication and in the capping of the 5' end of RNA genome. The specific aims include identifying the inherent catalytic activities of this viral protein, analyzing the functions of the conserved amino acids, and investigating the interactions between replicase and the RNA transcripts. The other ongoing project is to study the structure-function relationship of 6-phosphoglucose isomerase. Besides being a metabolic enzyme, catalyzing the inter-conversion between phosphoglucose and phosphofructose, this protein is also identical to neuroleukin and tumor autocrine motility factor in mammals. To study the catalytic mechanism of this enzyme, the enzymes from Bacillus stearothermophilus are subjected to structural analysis by X-ray crystallography and to functional analysis by mutagenesis. By combing the data from both approaches, we hope that the molecular action of 6-phosphoglucose isomerase can be appreciated in the near future.
Publication (1990-2002) :
Hong TY, Cheng CW, Huang JW, Meng M. Isolation and biochemical characterization of an endo-1,3-beta-glucanase from Streptomyces sioyaensis containing a C-terminal family 6 carbohydrate-binding module that binds to 1,3-beta-glucan. Microbiology(UK). 2002, 148:1151-1159.(Abstract)(pdf)
Meng M, Lin H, Hsieh C, Chen Y. Functions of the conserved anionic amino acids and those interacting with the substrate phosphate group of phosphoglucose isomerase. FEBS Lett 2001; 499:11-14.(Abstract) Huang CY, Huang YL, Meng M, Hsu YH, Tsai CH. Sequences at the 3' Untranslated Region of Bamboo Mosaic Potexvirus RNA Interact with the Viral RNA-Dependent RNA Polymerase. J Virol 2001; 75:2818-2824.(Abstract) Li YI, Chen YJ, Hsu YH, Meng M. Characterization of the AdoMet-dependent guanylyltransferase activity that is associated with the N terminus of bamboo mosaic virus replicase. J Virol 2001; 75:782-8.(Abstract) Chou CC, Sun YJ, Meng M, Hsiao CD. The crystal structure of phosphoglucose isomerase/autocrine motility factor/neuroleukin complexed with its carbohydrate phosphate inhibitors suggests its substrate/receptor recognition. J Biol Chem 2000; 275:23154-60.(Abstract) Meng M, Chane TL, Sun YJ, Hsiao CD. Probing the location and function of the conserved histidine residue of phosphoglucose isomerase by using an active-site directed inhibitor N-bromoacetylethanolamine phosphate. Protein Science 1999; 8:2438-2443.(Abstract) Sun YJ, Chou CC, Chen WS, Wu RT, Meng M, Hsiao CD. The crystal structure of a multifunctional protein: phosphoglucose isomerase/ autocrine motility factor/ neuroleukin. Proc. Natl. Acad. Sci. USA 1999: 96:5412-5417.(Abstract) Meng M, Chen YT, Hsiao YY, Itoh Y, & Bagdasarian M. Mutational analysis of the conserved cationic residues of Bacillus stearothermophilus 6-phosphoglucose isomerase. European Eur J Biochem 1998; 257:500-505.(Abstract) Li YI, Cheng YM, Huang YL, Tsai CH, Hsu YH, & Meng M. Identification and characterization of the E. coli-expressed RNA-dependent RNA polymerase of bamboo mosaic virus. J Virol 1998; 72:10093-10099.(Abstract) Hsiao CD, Chou CC, Hsiao YY, Sun YJ, & Meng M. Expression, purification, and crystallization of two isozymes of 6-phosphoglucose isomerase of Bacillus stearothermophilus. J Struct Biol 1997; 120:196-200.(Abstract) Davie JR, Wynn RM, Meng M, Huang Y, Aalund G, Chuang DT, Lau KS. Expression and characterization of branched-chain a-keto acid dehydrogenase kinase from the rat. Is it a histidine-protein kinase? J Biol Chem 1995; 270:19861-19867.(Abstract) Meng M, Chuang DT. Site-directed mutagenesis and functional analysis of the active-site residues of the E2 component of bovine branched-chain a-keto acid dehydrogenase complex. Biochemistry 1994; 33:12879-12885.(Abstract) Meng M, Bagdasarian M, Zeikus JG. The role of active-site aromatic and polar residues in catalysis and substrate discrimination by xylose isomerase. Proc. Natl. Acad. Sci. USA 1993; 90:8459-8463. (Abstract) Meng M, Bagdasarian M, Zeikus JG. Thermal stabilization of xylose isomerase from Thermoanaerobacterium thermosulfurigenes. Bio/Technology 1993; 11:1157-1161. Meng M, Lee C, Bagdasarian M, Zeikus JG. Switching substrate preference of thermophilic xylose isomerase from D-xylose to D-glucose by redesigning the substrate binding pocket. Proc. Natl. Acad. Sci. USA 1991; 88:4015-4019.(Abstract) Lee C, Bagdasarian M, Meng M, Zeikus JG. Catalytic mechanism of xylose (glucose) isomerase from Clostridium thermosulfurigenes. J Biol Chem 1990; 265:19082-19090.(Abstract)
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