賴美津 教授
  • 美國羅德島大學博士(Ph.D., 1989)
  • 電子郵件:mclai@dragon.nchu.edu.tw
  • 電話:886-(4)-22840416 分機 612,613
  • 傳真 886-(4)-22874740
  • 實驗室位置:生命科學大樓 613 室
  • 簡要經歷:美國加州大學洛杉磯分校(UCLA)博士後研究員(1989-1992)


研究
  • 研究領域 :古生菌學、甲烷菌、極端環境微生物、滲透壓調控分子生理、生醫材料。
  • 研究主題：
    • 高鹽古生菌類(絕對厭氧甲烷菌與好氧極端高鹽菌)的滲透壓調控分子生理。
    • 本土絕對厭氧甲烷菌的純化與分類鑑定。
    • 新型甲烷古生菌質體與病毒的分析。
    • 極端環境微生物的應用。
  • 近期研究計劃：
    • 高鹽厭氧甲烷菌的抗鹽相容質betaine的運送分子機制與生合成酵素的分子調控。
    • 高鹽厭氧甲烷菌的質體分析與載體構築。
    • 高鹽古生菌類(絕對厭氧甲烷菌與好氧極端高鹽菌)的抗鹽基因篩選與分析。
    • 臺灣本土自然環境甲烷菌的純化與分類鑑定。
    • 古生菌型聚酯應用於生物醫學基材的開發。



著作目錄

A. 期刊論文 (Reference papers 2001~1991)

1. Mei-Chin Lai* and Chao-Jen Shih. 2000. Characterization of Methanococcus voltaei Strain P2F9701a, A new Methanogen Isolated from Estuarine Environment. Current Microbiology 42:432-437. (Original article; SCI Impact Factor 1.0)

2. Mei-Chin Lai* and Sheng-Chung Chen. (2001). Methanofollis aquaemaris sp. nov., a Methanogen Isolated from an Aquaculture Fishpond. International Journal of Systematic and Evolutionary Microbiology, In Press (accepted 3/2001). (Original article; SCI Impact Factor 3.503)

3. Mei-Chin Lai*, Tong-Yung Hong and Robert P. Gunsalus. 2000. Glycine Betaine Transport in the Obligate Halophilic Archaeon, Methanohalophilus portucalensis. Journal of Bacteriology 182: 5020-5024. (SCI Impact Factor 3.712; original article)

4. Mei-Chin Lai*, Chi-Ming Shu, Sheng-Chung Chen, Li-Jane Lai, Ming-Shing Chiou and Jack Jay Hua. 2000. Methanosarcina mazei Strain O1M9704, Methanogen with Novel Tubule Isolated from Estuarine Environment. Current Microbiology 41:15-20. (SCI Impact Factor 1.094; original article)

5. Mei-Chin Lai*, Daw-Renn Yang, and Ming-Jen Chuang. 1999. Regulatory Factors Associated with Synthesis of the Osmolyte Glycine Betaine in the Halophilic Methanoarchaeon Methanohalophilus portucalensis. Applied and Environmental Microbiology 65:828-833. (SCI Impact Factor 3.541; original article)

6. Mei-Chin Lai*, Chi-Ming Shu, Ming-Shing Chiou, Tong-Yung Hong, Ming-Jen Chuang and Jack Jay Hua. 1999. Characterization of Methanosarcina mazei N2M9705 Isolate from an Aquaculture Fishpond. Current Microbiology 39: 79-84. (SCI Impact Factor 1.094; original article)

7. Mei-Chin Lai and Richard W. Traxler. 1994. A couple two stage continuous fermentation for solvent production by Clostridium acetobutylicum. Enzyme and Microbial Technology 16: 1021-1025. (SCI Impact Factor 1.223; original article)

8. Mei-Chin Lai and R. P. Gunsalus. 1992. Glycine betaine and Potassium are the major compatible solutes in the extreme halophilic methanogens. Journal of Bacteriology 174: 7474-7477. (SCI Impact Factor 3.712; original article)

9. Mary F. Roberts, Mei-Chin Lai and Robert. P. Gunsalus. 1992. Biosynthetic Pathways of the Osmolytes N-acetyl- -lysine, -glutamine, and Betaine in Methanohalophilus strain FDFI Suggested by Nuclear Magnetic Resonance Analyses. Journal of Bacteriology. 175:6688-6693. (SCI Impact Factor 3.712; original article)

10. Diane E. Robertson, Mei-Chin Lai, Robert P. Gunsalus and Mary F. Roberts. 1992. Composition, Variation, and Dynamics of Major Compatible Solutes in Methanohalophilus strain FDFI. Applied and Environmental Microbiology 58:2438-2443. (SCI Impact Factor 3.541; original article)

11. Mei-Chin Lai and Kevin R. Sowers, Diane E. Robertson, Mary F. Roberts and Robert. P. Gunsalus. 1991. The Distribution of Compatible Solutes in the Halophilic Methanogenic Archaebacteria. Journal of Bacteriology 173:5352-5358. (SCI Impact Factor 3.712; original article)

B. 研討會論文 (Conference abstracts 2001~1998):

1. Yu, Ping-Hung and Mei-Chin Lai*. 2001.A rolling circle plasmid from halophilic methanoarchaeon Methanohalophilus mahii. I-53, In Abstracts of the 101st General Meeting of the Society of Microbiology. American Society for Microbiology, Washington, D.C.

2. Lin, Chih-Chien and Mei-Chin Lai*. 2001. Isolation and Characterization of a new Methanogen from an Aquaculture Fishpond. I-56, In Abstracts of the 101st General Meeting of the Society of Microbiology. American Society for Microbiology, Washington, D.C.

3. 吳琰奇、賴美津*。2001。GlycineN-methyltranssferase of halophilic methanoarchaea。第十六屆生物醫學聯合學術年會。

4. 陳聖中、賴美津。2000。 從海水養殖場純化出來的一株新的甲烷菌種-Methanofollis aquamarinus sp. nov.。 中華民國微生物學會第三十四次大會。

5. 賴美津、余秉宏。2000。 從中度嗜鹽甲烷古生菌Methanohalophilus mahii分離之滾環型質體pML。中華民國微生物學會第三十四次大會。

6. 賴美津、林志鍵。2000。 自海水養殖場純化的甲烷菌Methanocalculus strain K1F9705b之分類鑑定與特性分析。中華民國微生物學會第三十四次大會。

7. Chia-Chi Wang and Mei-Chin Lai*. 2000. Methanosarcina mazei FA9604c, a new methanogenic strain isolated from an Upflow Anaerobic Sludge Blanket Reactor. I-18, In Abstracts of the 100th General Meeting of the Society of Microbiology. American Society for Microbiology, Washington, D. C.

8. Sheng-Chung Chen and Mei-Chin Lai*. 2000. Methanofollis strain N2F9704, a Novel Methanogen Isolated from an Aquaculture Fishpond That Grow as a Group inside a Sack, I-19, In Abstracts of the 100th General Meeting of the Society of Microbiology. American Society for Microbiology, Washington, D. C.

9. Chau-Rern Shy and Mei-Chin Lai*. 2000. Isolation and Characterization of A New Methanogenic Isolate from Estuarine Environment, I-20, In Abstracts of the 100th General Meeting of the Society of Microbiology. American Society for Microbiology, Washington, D. C.

10. Mei-Chin Lai*. 1999. Glycine N-Methyltransferase in the halophilic methanogen. Presented as a poster in Archaea: Ecology, Metabolism, and Molecular Biology section of Gordon Research Conferences at Andov, NH.

11. Mei-Chin Lai*, Daw-Renn Yang and Ming-Jen Chuang. 1999. Activity and Purification of Glycine N-methyltransferase in Halophilic Methanoarchaeon- Methanohalophilus portucalensis, I-17, In Abstracts of the 99th General Meeting of the Society of Microbiology. American Society foe Microbiology, Washington, D. C.

12. Sheng-Chung Chen and Mei-Chin Lai*. 1999. Characterization of two New Methanogenic isolates from Estuarine Environment, I-18, In Abstracts of the 99th General Meeting of the Society of Microbiology. American Society for Microbiology, Washington, D. C.

13. Hong, Tong-Yung and Mei-Chin Lai*. 1997. Glycine betaine transport in the halophilic methanogen, K-144, In Abstracts of the 97th General Meeting of the Society of Microbiology. American Society for Microbiology, Washington, D. C.

14. Mei-Chin Lai, Daw-Renn Yang and Ming-Jen Chuang. 1996. The biosynthesis and regulation of glycine betaine as compatible solutes in halophilic methanogens. Presented as a poster in Archaea: Ecology, Metabolism, and Molecular Biology section of Gordon Research Conferences at Plymouth, NH.

15. 賴麗娟，賴美津。1996。古生菌類抗鹽基因的轉殖與分析。中華民國微生物學會第三十次大會。

16. 莊旻真，賴美津。1996。嗜鹽性甲烷古生菌相容質glycine betaine生合成調控與其生合成酵素Glycine-N-methyltransferase的純化。中華民國微生物學會第三十次大會。

17. 洪堂耀，賴美津。1996。嗜鹽性甲烷古生菌運送相容質-glycine betaine機制之研究。中華民國微生物學會第三十次大會。

18. Mei-Chin Lai* and Daw-Renn Yang. 1995. Osmoregulation of Methanohalophilus: de novo biosynthesis of glycine betaine and its regulatory factors. Beijerinck Centennial. Microbial physiology and gene regulation: Emerging principles and application. The Hauge, The Netherlands.

19. 楊道任，賴美津。1994。 嗜鹽性甲烷菌之相容質-Glycine Betaine的生化合成及影響因子。中華民國微生物學會第二十八次大會。

20. Mei-Chin Lai. 1993. Plasmid in the halophilic methanogenic archaebacteria. Presented as a poster in the Methanogenesis section of Gordon Research Conferences at Plymouth, NH.

21. 賴美津。1993。 嗜鹽性甲烷古生菌的滲透壓調控機制。中華民國微生物學會第二十七次大會。

22. 廖采苓，賴美津。1993。 極端嗜鹽菌的分離存化及其特性的研究。中華民國微生物學會第二十七次大會。

23. Mei-Chin Lai and Robert P. Gunsalus. 1992. Glycine betaine and potassium are the major compatible solutes in the extreme halophilic methanogens, I-6, In Abstracts of the 92th General Meeting of the Society of Microbiology. American Society for Microbiology, Washington, D. C.

24. . Mei-Chin Lai, Kevin R. Sowers and Robert P. Gunsalus. 1991. Distribution of Compatible solutes in the Halophilic Methanogens, I-72, In Abstracts of the 91th General Meeting of the Society of Microbiology. American Society for Microbiology, Washington, D. C.

25. Mei-Chin Lai and Richard Traxler. 1990. Acid Assimilation Enzymes of Clostridium acetobutylicum and Its Spontaneous Degenerative Mutant, O-51, In Abstracts of the 90th General Meeting of the Society of Microbiology. American Society for Microbiology, Washington, D. C.

26. Mei-Chin Lai and Richard Traxler. 1990. The Degenerative Process and Degenerative Mutants of Clostridium acetobutylicum ATCC 824, O-52, In Abstracts of the 90th General Meeting of the Society of Microbiology. American Society for Microbiology, Washington, D. C.

C. 專書 (Books):
Mei-Chin Lai, R. Ciulla, M. F. Roberts, K. R. Sowers and R. P. Gunsalus. 1995. Extraction and detection of compatible intracellular solutes. In. Archaea: a laboratory manual (Tobb, F. T., Sowers K. R., DasSarma, S., Place, A. R., Schreier, H. J. and Fleischmann, E. M., Eds.). pp.349-368. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
D. 專利 (Patents):
賴美津, 徐善慧。 以極端嗜鹽古生菌生產新型聚酯 (Novel polyhydroxybutyrate by extreme halophilic archaea)。國科會審核通過辦理，中華民國專利申請中(申請案號89118071)。[NSC 88-2621-B-005-009-M08]
E. 基因序列寄存( New Gene Sequences Deposited in GenBank Database)

1. Lai*, M.-C. and C.C.Lin. 2001. Accession number: AF347025. Ribosomal RNA gene of Methanocalculus strain K1F9705b, 1254bp.

2. Yu, P.-H. and M.-C. Lai*. 2001. Accession number: AF321115. Ribosomal RNA gene of Methanocalculus train K1F9705c, 1251bp.

3. Lai*,M.-C. and Y.-C. Wu. 2001. Accession number:AY026256. Ribosomal RNA gene of Methanocalculus strain O1F9702C, 1254bp.

4. M. -C. Lai*, C. M. Shu, M. S. Chiou, M. J. Chuang, T.Y. Hong and J. J. Hua. 1999. Accession number: AF132849. Methanosarcina mazei strain N2M9705 16S ribosomal RNA gene, partial sequence.

5. M. S. Chiou and M. -C. Lai*. 1999. Accession number: AF153808. Methanosarcina mazei strain O1M9704 16S ribosomal RNA gene, partial sequence.

6. M. -C. Lai*, C. C. Wang, C. M. Shu, M. S. Chiou, L. J. Lai, M. J. Chuang, T.Y. Hong and J. J. Hua. 2000. Accession number: AF262036. Methanosarcina mazei strain FA9604C 16S ribosomal RNA gene, partial sequence.

7. M. -C. Lai*, S. C. Chen, C. M. Shu, M. S. Chiou and J. J. Hua. 2000. Accession number: AF262035. Methanofollis sp. N2F9704 16S ribosomal RNA gene, partial sequence.

8. M. -C. Lai*, C. M. Shu, S. C. Chen, M. S. Chiou, M. J. Chuang, T.Y. Hong and J. J. Hua. 2000. Accession number: AF172443. Methanocalculus taiwanense strain FA9604C 16S ribosomal RNA gene, partial sequence.

9. M.-C. Lai* and C.-J. Shih. 2000. Accession number: AF306670. Methanococcus sp. strain P2F9701a 16S ribosomal RNA gene, partial sequences.

10. M.-C. Lai* and P.-H. Yu. 2000. Accession number: AF321115. Methanocalculus sp. strain K1F9705c 16S ribosomal RNA gene, partial sequences.

F. 古生菌類菌株收集純化:
目前已純化本土甲烷菌十九株，其中十五株已寄存美國甲烷菌株中心(Oregon Collection of Methanogens)，編號分別為OCM #665，#666，#667，#668，#669，#670，#671，#672，#745，#746，#747，#748，#772，#755，#756。另純化極端高鹽菌十一株，其中一株Halobacterium strain H13已完成專利菌株寄存於食品工業發展研究所的菌種中心(編號CCRC 910151)，其餘十株尚未寄存。



開設課程
大學部:
微生物學、環境微生物學、太古生物學概論、微生物代謝與分子生理、生物技術等課程。
研究所:
微生物各論、微生物生理、古生菌學、極端微生物學及科學論文寫作等課程。