莊敦堯(Duen-yau Chuang)

Molecular and Cellular Biology, Biological Chemistry

學經歷	1.	學士:國立中興大學化學系 1990-1994
Experience	2.	研究生:日本東北大學、遺傳生態研究所 1994-1995
	3.	碩士:日本東北大學、遺傳子生態學專攻 1995-1997
	4.	博士:日本東北大學、遺傳子生態學專攻 1997-2000
	5.	研究員:日本東京大學、分子細胞生物學研究所 2000-2001
	6.	研究員:日本財團法人人類科學振興財團 2001-2002
	7.	國立中興大學化學系助理教授 2002~
通訊處 Contact Info.		chuang@dragon.nchu.edu.tw, 886-4-22840411 ext 817
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研究興趣
細胞分化機構與訊息傳遞, 基因選殖與表現, 基因轉錄調控機構解析

本研究室可分成三大方向:

1, 細胞分化機制研究
無論是人類或者是動物, 都是由一個受精卵變化而來。在胎兒的身體裡有許多具有分裂及分化的能力的細胞。這些同時具有分裂, 以及分化成其他具有特定功能組織的細胞我們稱為幹細胞(或前軀細胞)。然而這些帶有分化能力的細胞是如何被誘導分化的, 是那些因子影響這些細胞的前途的, 這些問題已隱然成為研究細胞生物學家的重要課題。本研究室主要是針對胎生肝細胞的分化機構的解析,希望能透過此一分化機構的了解上,找出適當的誘導因子去刺激這些幹細胞,使其分化成並能帶有表現肝臟的特有機能。
同時, 由於目前在許多組織裡（如骨隨, 脂肪等）可以發現幹細胞, 如何利用這些幹細胞則成為一項重要課題。本研究室也對這些研究方向深感興趣, 希望在當病患發生組織器官功障礙時,透過此一模式達到醫療上的貢獻。

2, 訊息傳遞與分化機構解析
由於身體內的各種組織細胞裡所表現的各種特有基因, 是藉由外來的訊息（如hormone, cytokine等）所活化的轉錄因子與該基因5'-flanking區域中的特定序列（element）結合而活化了該基因的表現。藉由對這些element的研究, 了解到當外來的訊息分子是如何的活化細胞內的特定轉錄蛋白, 經由這一連串的訊息傳遞機構的控制, 達到細胞內只表現特定基因的同時, 對其他非相關性基因無任何誘導能力。藉由對訊息傳遞機構的認識, 進而了解到這些element的利用時期,以及其表現的特定組織位置 (如只在肝臟裡被認識與表現), 並達到能進一步的利用這些element成為標的工具, 與我們想表現的基因結合, 使其僅在特定器官裡表現達到基因治療上的效果。

3, 細菌素機能與調控機構解析
細菌所生產的細菌素(抗生物質一種)只能殺死帶有近緣基因的細菌, 而對其他不相關的物種細胞無任何的抗生效果。這種帶有特異性活性抗生物質, 多數是由蛋白質所組成。本研究室主要是尋找新規的細菌素基因的尋找選殖, 並且這些新規的細菌素的殺菌活性部位、對近緣基因的細菌的辨識(受體)部位、細胞外進入細胞內的機構、更進一步的確認其反應機構及蛋白結構。此外, 細菌素調控機構也需要進一步的研究。藉由細菌素的特性的了解,以期可以應用在醫學, 工業, 農業, 分子生物學或各種生物防治上

Selected Publications:·
Articles
1. Kyeremeh, A.G., Kikumoto, T., Chuang, D.Y., Gunji, Y. and Ehara, Y. 1998. Evaluation of copper- and bactericide- resistance among strains of Erwinia carotovora subsp. carotovora. Ann. Phytopathol. Soc. Jpn. 64: 198-201.
2. Chuang, D.Y., Kyeremeh, A. G., Gunji, Y., Takahara, Y., Ehara, Y., and T. Kikumoto. 1999. Identification and Cloning of an Erwinia carotovora subsp. carotovora bacteriocin regulator gene by insertional mutagenesis. J. Bacteriol. 181:1953-1957.
3. Kyeremeh, A.G., Kikumoto, T., Chuang, D.Y., Gunji, Y. and Takahara, Y. 1999. Isolation and evaluation of copper- and bactericide-resistant mutants of putative biocontrol agents of soft rot of Chinese cabbage. Ann. Phytopathol. Soc. Jpn. 65: 171-176.
4. Kyeremeh, A.G., Kikumoto, T., Chuang, D.Y., Takahara, Y. and Ehara, Y. 1999. Studies on integration of bactericide-resistant biocontrol agents into conventional copper-bactericide sprays. Ann. Phytopathol. Soc. Jpn. 65: 571—575.
5. Kyeremeh, A.G., Kikumoto, T., Chuang, D.Y., Gunji, Y., Takahara, Y. and Ehara, Y. 2000. Biological Control of Soft Rot of Chinese Cabbage Using Single and Mixed Treatments of Bacteriocinproducing Avirulence Mutants of Erwinia carotovora subsp. Carotovora J. Gen. Plantpathol. 66:264-270.

Abstracts:
1. Kyeremeh, A.G., Chuang, D.Y., Gunji, Y., and Kikumoto, T. 1996. Copper-resistance in Erwinia carotovora subsp. Carotovora. Ann. Phytopathol. Soc. Jpn. 62: 605.
2. Kikumoto, T., Kyeremeh, A. G., Chuang, D. Y., and Gunji, Y. 1997. Biological control of the soft rot disease of Chinese cabbage with avirulent mutant strains of Erwinia carotovora subsp. Carotovora. Proc. 4th Intl. Workshop on PGPR, Japan-OECD Joint Workshop, Sapporo, pp. 118-119.
3. Kyeremeh, A.G., Chuang, D.Y., Gunji, Y., Takahara, Y., and Kikumoto, T. 1998. Isolation and Evaluation of Copper- and Bacteriocide- resistant Mutants of Putative Biocontrol Agents of the Soft-rot Disease of Chinese Cabbage. Ann. Phytopathol. Soc. Jpn. 64: 385.
4. Chuang, D.Y., Kyeremeh, A. G., Gunji, Y., Takahara, Y., and T. Kikumoto. 1998. Cloning of Bacteriocin Regulator gene (brg) from Erwinia carotovora subsp. Carotovora. Ann. Phytopathol. Soc. Jpn. 64:389-390.
5. Chuang, D.Y., Kyeremeh, A. G., and T. Kikumoto. 1998. Diverse Bacteriocins Produced by Erwinia carotovora subsp. Carotovora Ann. Phytopathol. Soc. Jpn. 64: 608-609.
6. Chuang, D.Y., Kyeremeh, A. G., and T. Kikumoto. 2000. Cloning and Expression of the Erwinia carotovora subsp. Carotovora Gene Encoding the Low-Molecular-Weight Bacteriocin, H1. Ann. Phytopathol. Soc. Jpn. 66: 138.
7. Chuang, D. Y., Matsui, T., Kinoshita, T., and Miyajima, A., 2001. Regulation of Tyrosine Amino Transferase Expression by Oncostatin M in Fetal Liver. Workshop of Hepatocytes, 2001.
8. Chuang, D. Y., Matsui, T., Kinoshita, T., and Miyajima, A. 2001. Regulation of Tyrosine Amino Transferase Expression by Oncostatin M in Fetal Liver. 4th IMCBNS, Taipei, Taiwan.
9. Chuang, D. Y., Matsui, T., Kinoshita, T., and Miyajima, A. 2001.Oncostatin M regulates expression of tyrosine amino transferase gene through STAT3 and C/EBPα. Ann. Mol. Biol. 2001.
10. Chuang, D. Y., Matsui, T., and Miyajima, A., 2001. Regulation of Tyrosine Amino Transferase Expression through STAT3 and C/EBPα in Fetal Liver. Workshop of Hepatocytes, 2002.

Preparation Articles
1. Chuang, D. Y., Kyeremeh, A. G., and Kikumoto, T. Induction of Bacteriocin Synthesis in Erwinia carotovora subsp. Carotovora is controlled by the expression of flhDC. (Preparation)
2. Chuang, D. Y., Kyeremeh, A. G., and Kikumoto, T. Cloning and Expression of Erwinia carotovora subsp. carotovora Gene Encoding the Low-Molecular-Weight Bacteriocin, Carocin H1. (Preparation)
3. Chuang, D. Y., and Kikumoto, T. New Induction and Regulatory Mechanism of the Low-Molecular-Weight Bacteriocin Expression in Erwinia carotovora subsp. carotovora. (Preparation)
4. Chuang, D. Y., Matsui, T., and Miyajima, A. Cooperation of STAT3 and C/EBPα for expression of the tyrosine amino transferase gene in mouse fetal hepatocytes: identification of an Oncostatin M responsive element. (Preparation)